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PMID: 6305908 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Ti plasmid and chromosomal ornithine catabolism genes of Agrobacterium tumefaciens C58.

Journal of bacteriology ·Vol. 155 ·No. 1 ·1983-07-00 ·Pages 196-202

Schardl CL, Kado CI

Abstract

The pTiC58 plasmid noc genes of Agrobacterium tumefaciens C58 code for nopaline oxidase (nocC), nopaline permease (nocP), the inducible periplasmic protein n1 (nocB), and a function(s) required for ornithine catabolism (nocA). In addition, strains C58 and Ach-5 of A. tumefaciens have chromosomal ornithine catabolism genes. The chromosomal orc gene codes for ornithine dehydrogenase. Strain C58 is normally orc, but orc+ mutants can be selected. We have characterized both chromosomal orc and pTiC58 nocA plasmid genes. Complementation of most chromosomal orc mutants by pTiC58 restored growth on both nopaline and L-ornithine but did not restore ornithine dehydrogenase activity. We conclude that ornithine is an intermediate of nopaline degradation and that the Ti plasmid and chromosome both code for ornithine-degradative enzymes. A model for nopaline catabolism is presented.

MeSH Terms
Bacterial Proteins/genetics Chromosomes, Bacterial/physiology Genes Genes, Bacterial Kinetics Membrane Transport Proteins/genetics Mutation Ornithine/metabolism Oxidoreductases Acting on CH-NH Group Donors/genetics Phenotype Plasmids Rhizobium/genetics,metabolism Species Specificity
Chemicals
Bacterial Proteins Membrane Transport Proteins NocP protein, Agrobacterium tumefaciens Ornithine Oxidoreductases Acting on CH-NH Group Donors nopaline dehydrogenase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schardl C L
Kado C I
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23 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.155.1.196-202.1983
Published
1983-07-00
Pages
196-202
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217669
Subset
IM
Grants
NCI NIH HHS · CA-11526 · United States
External Links
PubMed source DOI: 10.1128/jb.155.1.196-202.1983 PMC Full Text View journal details
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