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PMID: 6304702 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequence specificity of actinomycin D and Netropsin binding to pBR322 DNA analyzed by protection from DNase I.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 11 ·1983-06-00 ·Pages 3260-4

Lane MJ, Dabrowiak JC, Vournakis JN

Abstract

A direct approach to determining the sequence specificities of equilibrium binding drugs by using the DNase protection technique is described. The method utilizes singly end-labeled restriction fragments and partial digestion of the drug fragment complex with DNase I. Microdensitometry of autoradiograms produced after electrophoretic separation of digestion products allows determination of sequences that are affected by drug binding. The feasibility of the technique for locating small ligands bound to DNA and its eventual use as a quantitative thermodynamic approach to studying ligand binding to heterogeneous DNA as a function of sequence is illustrated by using actinomycin D and Netropsin.

MeSH Terms
Animals Autoradiography Base Sequence Cattle Chemical Phenomena Chemistry DNA Dactinomycin Deoxyribonuclease I Endodeoxyribonucleases/metabolism Guanidines Netropsin Phosphorus Radioisotopes Plasmids Substrate Specificity Thymus Gland
Chemicals
Guanidines Phosphorus Radioisotopes Dactinomycin Netropsin DNA Endodeoxyribonucleases Deoxyribonuclease I
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lane M J
Dabrowiak J C
Vournakis J N
References (21)
21 references, click to expand
  1. On the mechanism of action of bleomycin: scission of DNA strands in vitro and in vivo.
    J Antibiot (Tokyo). 1969 Sep;22(9):446-8 PMID: 4982665
  2. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.
    J Bacteriol. 1972 May;110(2):667-76 PMID: 4336693
  3. Association of actinomycin D and deoxyribodinucleotides as a model for binding of the drug to DNA.
    Proc Natl Acad Sci U S A. 1972 Jul;69(7):1911-4 PMID: 4505669
  4. Stereochemistry of actinomycin binding to DNA. II. Detailed molecular model of actinomycin-DNA complex and its implications.
    J Mol Biol. 1972 Jul 14;68(1):21-34 PMID: 4115109
  5. Effects of the antibiotics netropsin and distamycin A on the structure and function of nucleic acids.
    Prog Nucleic Acid Res Mol Biol. 1975;15(0):285-318 PMID: 1094496
  6. Stopped-flow kinetic studies of actinomycin binding to DNAs.
    Biochemistry. 1975 May 20;14(10):2138-45 PMID: 50082
  7. Transmission of stability (telestability) in deoxyribonucleic acid. Physical and enzymatic studies on the duplex block polymer d(C15A15) - d(T15G15).
    J Biol Chem. 1975 Jul 10;250(13):5109-13 PMID: 50320
  8. Netropsin-poly(dA-dT) complex in solution: structure and dynamics of antibiotic-free base pair regions and those centered on bound netropsin.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5207-11 PMID: 271945
  9. DNAse footprinting: a simple method for the detection of protein-DNA binding specificity.
    Nucleic Acids Res. 1978 Sep;5(9):3157-70 PMID: 212715
  10. The interaction of RNA polymerase and lac repressor with the lac control region.
    Nucleic Acids Res. 1979 Jan;6(1):111-37 PMID: 370784
  11. Complete nucleotide sequence of the Escherichia coli plasmid pBR322.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:77-90 PMID: 383387
  12. Fractionation of DNA fragments by polyethylene glycol induced precipitation.
    Methods Enzymol. 1980;65(1):347-53 PMID: 6246357
  13. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  14. The binding of a transcription factor to deletion mutants of a 5S ribosomal RNA gene.
    Cell. 1981 Mar;23(3):665-9 PMID: 7226225
  15. Mapping the topography of DNA wrapped around gyrase by nucleolytic and chemical probing of complexes of unique DNA sequences.
    Cell. 1981 Mar;23(3):721-9 PMID: 6261954
  16. Equilibrium binding of carcinogens and antitumor antibiotics to DNA: site selectivity, cooperativity, allosterism.
    Nucleic Acids Res. 1981 Jul 10;9(13):3175-86 PMID: 6456451
  17. Strand scission of deoxyribonucleic acid by neocarzinostatin, auromomycin, and bleomycin: studies on base release and nucleotide sequence specificity.
    Biochemistry. 1981 Dec 22;20(26):7599-606 PMID: 6173064
  18. Altered mobility of polydeoxyribonucleotides in high resolution polyacrylamide gels due to removal of terminal phosphates.
    Nucleic Acids Res. 1981 Dec 21;9(24):6787-94 PMID: 7335493
  19. Mutual interaction between adjacent dG . dC actinomycin binding sites and dA . dT netropsin binding sites on the self-complementary d(C-G-C-G-A-A-T-T-C-G-C-G) duplex in solution.
    Proc Natl Acad Sci U S A. 1981 Dec;78(12):7281-4 PMID: 6278465
  20. DNA conformation, dynamics, and interactions in solution.
    Science. 1982 May 7;216(4546):581-90 PMID: 6280281
  21. Map of distamycin, netropsin, and actinomycin binding sites on heterogeneous DNA: DNA cleavage-inhibition patterns with methidiumpropyl-EDTA.Fe(II).
    Proc Natl Acad Sci U S A. 1982 Sep;79(18):5470-4 PMID: 6291045
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.11.3260
Published
1983-06-00
Pages
3260-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC394020
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.80.11.3260 PMC Full Text View journal details
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