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PMID: 6302677 Published · ppublish English Journal Article

Association of pp36, a phosphorylated form of the presumed target protein for the src protein of Rous sarcoma virus, with the membrane of chicken cells transformed by Rous sarcoma virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 4 ·1983-02-00 ·Pages 960-4

Amini S, Kaji A

Abstract

A cellular protein with a molecular mass of approximately 36 kilodaltons is the presumed target protein of the src protein [the transforming protein encoded by Rous sarcoma virus (RSV)]. The cellular location of the phosphorylated 36-kilo-dalton protein (pp36) in chicken embryo fibroblasts transformed by the Schmidt-Ruppin strain of RSV has been investigated. In these studies, two-dimensional electrophoresis was used for detection of the phosphoproteins in total cell extracts and also in fractionated subcellular components. We conclude that pp36 is localized in the plasma membrane, on the basis of the following observations. (i) Fractionation of 32P-labeled cell extracts showed that pp36 is almost exclusively localized in the crude membrane fraction and no appreciable amount was found in nuclear or cytoplasmic fractions. (ii) On further fractionation of the crude membrane fraction, pp36 was localized mostly in the plasma membrane rather than in other membranous fractions. (iii) Isolated plasma membrane by itself phosphorylated the 36-kilodalton protein on incubation with [gamma-32P]ATP.

MeSH Terms
Animals Avian Sarcoma Viruses/physiology Cell Membrane/metabolism Cell Nucleus/metabolism Cell Transformation, Viral Cells, Cultured Chickens Cytoplasm/metabolism Isoelectric Point Molecular Weight Phosphoproteins/metabolism
Chemicals
Phosphoproteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Amini S
Kaji A
References (37)
37 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.4.960
Published
1983-02-00
Pages
960-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393507
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.80.4.960 PMC Full Text View journal details
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