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PMID: 6300874 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Inhibition of Micrococcus luteus DNA topoisomerase I by UV photoproducts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 7 ·1983-04-00 ·Pages 1787-91

Pedrini AM, Ciarrocchi G

Abstract

The activity of Micrococcus luteus DNA topoisomerase I on UV-irradiated supercoiled DNA was studied under either processive or distributive reaction conditions. Changes in DNA structure caused by UV irradiation reduce the rate of DNA relaxation at very low concentration of photoproducts. Under processive conditions the inhibition of the topoisomerase I by photoproducts can be quantitated by measuring the amount of substrate left in the replicative form I band. The mode of action of DNA topoisomerase I was affected by the presence of photoproducts in the DNA substrate, although the ability of the enzyme to form a covalent complex with UV-irradiated supercoiled DNA was not changed. The inhibition of topoisomerase I by UV photoproducts has been compared to the effects of single-stranded DNA and UV-irradiated duplex linear DNA on the enzyme, and the results suggest that the inhibition by photoproducts is caused by changes in the conformation of the supercoil. Our findings indicate the possibility that DNA topoisomerase I plays a role in repair.

MeSH Terms
DNA, Superhelical/radiation effects Micrococcus/enzymology Nucleic Acid Conformation Plasmids Pyrimidine Dimers/physiology Structure-Activity Relationship Topoisomerase I Inhibitors Ultraviolet Rays
Chemicals
DNA, Superhelical Pyrimidine Dimers Topoisomerase I Inhibitors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pedrini A M
Ciarrocchi G
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.7.1787
Published
1983-04-00
Pages
1787-91
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393694
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.80.7.1787 PMC Full Text View journal details
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