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PMID: 6298597 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

DNA sequence analysis of a mouse pro alpha 1 (I) procollagen gene: evidence for a mouse B1 element within the gene.

Molecular and cellular biology ·Vol. 2 ·No. 11 ·1982-11-00 ·Pages 1362-71

Monson JM, Friedman J, McCarthy BJ

Abstract

In a 3.8-kilobase mouse DNA sequence encoding amino acid sequences for the pro alpha 1(I) chain of type I procollagen, 14 coding sequences were identified which specify a sequence 95% homologous to amino acid residues 568 to 963 of the bovine alpha 1(I) chain. All of these coding sequences were flanked by appropriate splice junctions following the GT/AG rule. These observations suggest, but do not prove, that this pro alpha 1(I) gene is transcriptionally active. Of the 14 coding sequences, 7 were 54 base pairs in length, whereas the remainder were higher multiples of 54 base pairs. Nonrandom utilization of codons pertained throughout all of the coding sequences showing a preference (56%) for U in the wobble position. Two of the intervening sequences encoded imperfect vestiges of coding sequences which exhibited a codon preference different from that of the pro alpha 1(I) gene proper and were not flanked by splice junctions. One intervening sequence encoded a member of the mouse B1 family of middle repetitive sequences. It was flanked by 8-base-pair direct repeats and had a truncated A-rich region, suggesting that it may be a mobile element. Within this element were sequences which could function as a RNA polymerase III split promoter.

MeSH Terms
Animals Base Sequence Codon DNA DNA Restriction Enzymes Genes Mice Nucleic Acid Hybridization Procollagen/genetics RNA Splicing Repetitive Sequences, Nucleic Acid Transcription, Genetic
Chemicals
Codon Procollagen DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Monson J M
Friedman J
McCarthy B J
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.2.11.1362-1371.1982
Published
1982-11-00
Pages
1362-71
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369941
Subset
IM
Grants
NIADDK NIH HHS · AM 25157A · United States
Databases
GENBANK
M17491, M17492, M17493
External Links
PubMed source DOI: 10.1128/mcb.2.11.1362-1371.1982 PMC Full Text View journal details
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