Home LiteratureArticle Details
PMID: 6296830 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A DNA-mediated transformation system for Dictyostelium discoideum.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 23 ·1982-12-00 ·Pages 7356-60

Hirth KP, Edwards CA, Firtel RA

Abstract

We have established a transformation system for Dictyostelium discoideum. The transformation vector contains the protein coding region of the Tn5 neomycin resistance gene fused to the proposed promoter of the Dictyostelium actin 8 gene; the vector also contains a sequence that acts as an autonomously replicating sequence (ars) in yeast. Using this vector, we can transform Dictyostelium vegetative amoebae to be resistant to aminoglycoside G418 at a frequency of between 10(-6) and 10(-4) of the input cells. The transformed cell lines are stable and contain vector sequences integrated within chromosomal DNA.

MeSH Terms
Aminoglycosides/pharmacology DNA/genetics DNA Transposable Elements Dictyostelium/genetics Drug Resistance Genetic Vectors Neomycin/pharmacology Transformation, Genetic
Chemicals
Aminoglycosides DNA Transposable Elements DNA Neomycin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hirth K P
Edwards C A
Firtel R A
References (24)
24 references, click to expand
  1. Sensitivity of Dictyostelium discoideum to nucleic acid analogues.
    Exp Cell Res. 1971 Feb;64(2):484-6 PMID: 5542656
  2. RNA in cytoplasmic and nuclear fractions of cellular slime mold amebas.
    J Cell Biol. 1970 May;45(2):399-407 PMID: 5535143
  3. Characterization of the genome of the cellular slime mold Dictyostelium discoideum.
    J Mol Biol. 1972 May 28;66(3):339-61 PMID: 4556574
  4. Structural organization of the genome of Dictyostelium discoideum: analysis by EcoR1 restriction endonuclease.
    J Mol Biol. 1976 Apr 25;102(4):831-52 PMID: 781265
  5. Transformation of yeast.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):1929-33 PMID: 347451
  6. Identification and analysis of Dictyostelium actin genes, a family of moderately repeated genes.
    Cell. 1978 Nov;15(3):763-78 PMID: 728989
  7. Dictyostelium rDNA consists of non-chromosomal palindromic dimers containing 5S and 36S coding regions.
    Chromosoma. 1978 Dec 21;70(1):19-29 PMID: 738167
  8. High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1035-9 PMID: 375221
  9. Replication in Saccharomyces cerevisiae of plasmid pBR313 carrying DNA from the yeast trpl region.
    Gene. 1979 Oct;7(2):141-52 PMID: 389741
  10. Analysis of the multigene family coding the developmentally regulated carbohydrate-binding protein discoidin-I in D. discoideum.
    Cell. 1980 Jun;20(2):495-505 PMID: 7388948
  11. Expression of a transposable antibiotic resistance element in Saccharomyces.
    Nature. 1980 Oct 30;287(5785):869-71 PMID: 6253817
  12. Eukaryotic DNA segments capable of autonomous replication in yeast.
    Proc Natl Acad Sci U S A. 1980 Aug;77(8):4559-63 PMID: 6449009
  13. Transforming DNA integrates into the host chromosome.
    Cell. 1981 Jan;23(1):29-39 PMID: 7214526
  14. A change in the rate of transcription of a eukaryotic gene in response to cyclic AMP.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7171-5 PMID: 6261244
  15. Synthesis and stability of developmentally regulated dictyostelium mRNAs are affected by cell--cell contact and cAMP.
    Cell. 1981 Jun;24(3):785-97 PMID: 6265103
  16. Differential expression and 5' end mapping of actin genes in Dictyostelium.
    Cell. 1981 Jun;24(3):799-807 PMID: 6894715
  17. Inhibition of SV40 replication in simian cells by specific pBR322 DNA sequences.
    Nature. 1981 Sep 3;293(5827):79-81 PMID: 6267479
  18. A new dominant hybrid selective marker for higher eukaryotic cells.
    J Mol Biol. 1981 Jul 25;150(1):1-14 PMID: 6271971
  19. Cell patterning in Dictyostelium.
    Philos Trans R Soc Lond B Biol Sci. 1981 Oct 7;295(1078):497-508 PMID: 6117907
  20. Yeast transformation: a model system for the study of recombination.
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6354-8 PMID: 6273866
  21. Evidence for sub-families of actin genes in Dictyostelium as determined by comparisons of 3' end sequences.
    J Mol Biol. 1981 Oct 5;151(4):593-606 PMID: 6276562
  22. Sequence and expression of the discoidin I gene family in Dictyostelium discoideum.
    J Mol Biol. 1981 Dec 5;153(2):273-89 PMID: 6279874
  23. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.
    J Mol Appl Genet. 1982;1(4):327-41 PMID: 6286831
  24. Two separate regions of the extrachromosomal ribosomal deoxyribonucleic acid of Tetrahymena thermophila enable autonomous replication of plasmids in Saccharomyces cerevisiae.
    Mol Cell Biol. 1981 Jun;1(6):535-43 PMID: 6765606
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.23.7356
Published
1982-12-00
Pages
7356-60
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC347338
Subset
IM
Grants
NIGMS NIH HHS · 2ROI GM24279-05 · United States
NIGMS NIH HHS · GM-07240 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.23.7356 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2