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PMID: 6296776 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Effect of rifampicin on expression of lacZ fused to promoters or terminators of the E.coli rpoBC operon.

Nucleic acids research ·Vol. 10 ·No. 22 ·1982-11-25 ·Pages 7425-38

Howe KM, Newman AJ, Garner I, Wallis A, Hayward RS

Abstract

The genes encoding the beta and beta' subunits of RNA polymerase in E.coli lie downstream of at least two ribosomal protein genes in a single unit of transcription. Treatment of E.coli with rifampicin, under conditions producing partial inhibition of general RNA synthesis, can strongly stimulate transcription of the polymerase genes, while activating the neighbouring ribosomal genes only slightly. We have investigated the mechanism of this effect by fusing strong promoters, a weak internal promoter, and an attenuator of the polymerase operon to the lacZ gene, in derivatives of plasmid pMC81. Studies of these fusions confirm our conclusion, based on similar fusions to galK, that rifampicin can foster readthrough of transcriptional terminators. They also suggest the existence of extra terminators and anti-termination elements in the above transcription unit.

MeSH Terms
DNA Restriction Enzymes DNA-Directed RNA Polymerases/genetics Escherichia coli/drug effects,genetics Genes/drug effects Lac Operon/drug effects Macromolecular Substances Operon/drug effects Ribosomal Proteins/genetics Rifampin/pharmacology Transcription, Genetic/drug effects beta-Galactosidase/genetics
Chemicals
Macromolecular Substances Ribosomal Proteins DNA-Directed RNA Polymerases DNA Restriction Enzymes beta-Galactosidase Rifampin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Howe K M
Newman A J
Garner I
Wallis A
Hayward R S
References (25)
25 references, click to expand
  1. Isolation of a specialized lambda transducing bacteriophage carrying the beta subunit gene for Escherichia coli ribonucleic acid polymerase.
    J Bacteriol. 1973 Nov;116(2):517-26 PMID: 4583237
  2. Different effects of rifampicin and streptolydigin on the control of RNA polymerase subunit synthesis in Escherichia coli.
    Mol Gen Genet. 1974;134(2):181-6 PMID: 4617163
  3. Induction of sigma factor synthesis in Escherichia coli by the N gene product of bacteriophage lambda.
    Proc Natl Acad Sci U S A. 1976 Dec;73(12):4405-9 PMID: 794877
  4. Lambdoid phages that simplify the recovery of in vitro recombinants.
    Mol Gen Genet. 1977 Jan 7;150(1):53-61 PMID: 319344
  5. Mapping of a cluster of genes for components of the transcriptional and translational machineries of Escherichia coli.
    J Mol Biol. 1977 Jan 5;109(1):23-47 PMID: 320344
  6. Hybridization mapping of restriction fragments from the early region of bacteriophage T7 DNA.
    Virology. 1977 Oct 15;82(2):275-87 PMID: 919341
  7. Transcription patterns of adjacent segments on the chromosome of Escherichia coli containing genes coding for four 50S ribosomal proteins and the beta and beta' subunits of RNA polymerase.
    J Mol Biol. 1977 Oct 5;115(4):603-25 PMID: 338915
  8. Non-coordinate expression of the neighbouring genes rplL and rpoB,C of Escherichia coli.
    Mol Gen Genet. 1978 Mar 20;160(1):77-80 PMID: 347250
  9. Control of transcription termination.
    Annu Rev Biochem. 1978;47:967-96 PMID: 354508
  10. Gene expression in Escherichia coli B/r during partial rifampicin-mediated restrictions of transcription initiation.
    Mol Gen Genet. 1978 Sep 20;165(1):79-86 PMID: 362168
  11. DNA sequence of the araBAD promoter in Escherichia coli B/r.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4724-8 PMID: 368797
  12. Nucleotide sequence of the ribosomal protein gene cluster adjacent to the gene for RNA polymerase subunit beta in Escherichia coli.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1697-701 PMID: 377281
  13. Transcriptional and post-transcriptional control of RNA polymerase and ribosomal protein genes cloned on composite ColE1 plasmids in the bacterium Escherichia coli.
    J Biol Chem. 1979 Aug 25;254(16):7540-7 PMID: 381287
  14. Control features within the rplJL-rpoBC transcription unit of Escherichia coli.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):4922-6 PMID: 116224
  15. Genetics of bacterial RNA polymerases.
    Annu Rev Genet. 1979;13:59-97 PMID: 94253
  16. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.
    J Mol Biol. 1980 Apr;138(2):179-207 PMID: 6997493
  17. Attenuation and processing of RNA from the rplJL--rpoBC transcription unit of Escherichia coli.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3331-5 PMID: 6158044
  18. Regulation of biosynthesis of the DNA-dependent RNA polymerase in Escherichia coli.
    Curr Top Cell Regul. 1980;17:89-136 PMID: 7002486
  19. Characterization of promoter-cloning plasmids: analysis of operon structure in the rif region of Escherichia coli and isolation of an enhanced internal promoter mutant.
    J Bacteriol. 1980 Dec;144(3):904-16 PMID: 7002914
  20. Nucleotide sequence of the trpB gene in Escherichia coli and Salmonella typhimurium.
    J Mol Biol. 1980 Oct 5;142(4):489-502 PMID: 7007651
  21. Translational coupling during expression of the tryptophan operon of Escherichia coli.
    Genetics. 1980 Aug;95(4):785-95 PMID: 6162715
  22. A direct effect of guanosine tetraphosphate on pausing of Escherichia coli RNA polymerase during RNA chain elongation.
    J Biol Chem. 1981 Mar 25;256(6):2787-97 PMID: 7009598
  23. E. coli ribosomal protein L10 inhibits translation of L10 and L7/L12 mRNAs by acting at a single site.
    Nature. 1981 Nov 12;294(5837):190-2 PMID: 6272122
  24. In vivo synthesis of a polycistronic messenger RNA for the ribosomal proteins L11, L1, L10 and L7/12 in Escherichia coli.
    Mol Gen Genet. 1981;183(2):277-82 PMID: 7035827
  25. Expression of ribosomal genes in bacteria.
    Adv Genet. 1982;21:53-121 PMID: 7036693
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.22.7425
Published
1982-11-25
Pages
7425-38
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327015
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.22.7425 PMC Full Text View journal details
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