Transcription of herpes simplex virus genes in vivo: overlap of a late promoter with the 3' end of the early thymidine kinase gene.
We identified in herpes simplex virus type 1-infected cells six cytoplasmic transcripts which were complementary to BamHI restriction endonuclease fragment Q. Two transcripts appeared in major amounts compared with the other four. One major transcript of about 1.4 kilobases was the mRNA for the viral thymidine kinase, was synthesized at intermediate times, and was classified as a beta transcript. The other major transcript was synthesized at late times and was classified as a gamma transcript. This late transcript was about 3 kilonucleotides long and was transcribed in the same direction as the gene for thymidine kinase. The 5' end of this late RNA was located by RNA sequence analysis and was 23 nucleotides downstream from the polyadenylation site for the thymidine kinase mRNA. This finding led to the conclusion that the control region for the 3-kilobase gamma transcript is contained within the 3' untranslated region of the thymidine kinase transcript.
26 references, click to expand
-
Regulation of herpesvirus macromolecular synthesis. I. Cascade regulation of the synthesis of three groups of viral proteins.J Virol. 1974 Jul;14(1):8-19 PMID: 4365321
-
Preparation and preliminary characterization of purified ovalbumin messenger RNA from the hen oviduct.Biochemistry. 1975 Jan 14;14(1):69-78 PMID: 1109593
-
Secondary structure maps of ribosomal RNA. II. Processing of mouse L-cell ribosomal RNA and variations in the processing pathway.J Mol Biol. 1974 Oct 25;89(2):397-407 PMID: 4475117
-
A restriction fragment analysis of the T7 left-early region.Virology. 1975 Dec;68(2):360-73 PMID: 1081780
-
A new method for sequencing DNA.Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
-
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
-
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.Cell. 1977 Nov;12(3):721-32 PMID: 922889
-
Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8 PMID: 73185
-
Characterization of the mRNA for herpes simplex virus thymidine kinase by cell-free synthesis of active enzyme.Nucleic Acids Res. 1978 Jul;5(7):2333-44 PMID: 209407
-
Rapid sequence determination of late simian virus 40 16S mRNA leader by using inhibitors of reverse transcriptase.Proc Natl Acad Sci U S A. 1979 Feb;76(2):731-5 PMID: 85304
-
Separation and characterization of herpes simplex virus type 1 immediate-early mRNA's.J Virol. 1979 Jul;31(1):42-52 PMID: 228058
-
Construction and characterization of a recombinant plasmid encoding the gene for the thymidine kinase of Herpes simplex type 1 virus.Gene. 1979 Nov;7(3-4):335-42 PMID: 230124
-
Viral DNA synthesis is required for the efficient expression of specific herpes simplex virus type 1 mRNA species.Virology. 1980 Feb;101(1):10-24 PMID: 6243816
-
Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper.Methods Enzymol. 1979;68:220-42 PMID: 94421
-
A herpes simplex virus type 1 function continuously required for early and late virus RNA synthesis.Nature. 1980 May 29;285(5763):329-30 PMID: 6246451
-
The association of herpes simplex virus with squamous carcinoma of the cervix, and studies of the virus thymidine kinase gene.Proc R Soc Lond B Biol Sci. 1980 Nov 19;210(1180):411-21 PMID: 6109302
-
The nucleotide sequence and transcript map of the herpes simplex virus thymidine kinase gene.Nucleic Acids Res. 1980 Dec 20;8(24):5949-64 PMID: 6258156
-
Adenovirus early gene products may control viral mRNA accumulation and translation in vivo.Cell. 1981 Feb;23(2):485-96 PMID: 7471209
-
Structures of two spliced herpes simplex virus type 1 immediate-early mRNA's which map at the junctions of the unique and reiterated regions of the virus DNA S component.J Virol. 1981 Jan;37(1):431-44 PMID: 6260993
-
Detailed characterization of the mRNA mapping in the HindIII fragment K region of the herpes simplex virus type 1 genome.J Virol. 1981 Mar;37(3):1011-27 PMID: 6262521
-
Nucleotide sequence of the thymidine kinase gene of herpes simplex virus type 1.Proc Natl Acad Sci U S A. 1981 Mar;78(3):1441-5 PMID: 6262799
-
The sequence 5'-AAUAAA-3'forms parts of the recognition site for polyadenylation of late SV40 mRNAs.Cell. 1981 Apr;24(1):251-60 PMID: 6113054
-
Characterization of a major late herpes simplex virus type 1 mRNA.J Virol. 1981 May;38(2):483-96 PMID: 6264123
-
Organization and expression of eucaryotic split genes coding for proteins.Annu Rev Biochem. 1981;50:349-83 PMID: 6791577
-
Herpes simplex virus type 1 HindIII fragment L encodes spliced and complementary mRNA species.J Virol. 1981 Aug;39(2):559-72 PMID: 6268843
-
In vitro transcription of the thymidine kinase gene of herpes simplex virus.Proc Natl Acad Sci U S A. 1982 Sep;79(17):5215-9 PMID: 6291032
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