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PMID: 6264123 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of a major late herpes simplex virus type 1 mRNA.

Journal of virology ·Vol. 38 ·No. 2 ·1981-05-00 ·Pages 483-96

Costa RH, Devi BG, Anderson KP, Gaylord BH, Wagner EK

Abstract

A major, late 6-kilobase (6-kb) mRNa mapping in the large unique region of herpes simplex virus type 1 (HSV-1) was characterized by using two recombinant DNA clones, one containing EcoRI fragment G (0.190 to 0.30 map units) in lambda. WES.B (L. Enquist, M. Madden, P. Schiop-Stansly, and G. Vandl Woude, Science 203:541-544, 1979) and one containing HindIII fragment J (0.181 to 0.259 map units) in pBR322. This 6-kb mRNA had its 3' end to the left of 0.231 on the prototypical arrangement of the HSV-1 genome and was transcribed from right to left. It was bounded on both sides by regions containing a large number of distinct mRNA species, and its 3' end was partially colinear with a 1.5-kb mRNA which encoded a 35,000-dalton polypeptide. The 6-kb mRNA encoded a 155,000-dalton polypeptide which was shown to be the only one of this size detectable by hybrid-arrested translation encoded by late polyadenylated polyribosomal RNA. The S1 nuclease mapping experiments indicated that there were no introns in the coding sequence for this mRNA and that its 3' end mapped approximately 800 nucleotides to the left of the BglII site at 0.231, whereas its 5' end extended very close to the BamHI site at 0.266.

MeSH Terms
Base Sequence DNA Restriction Enzymes DNA, Recombinant Genes, Viral Protein Biosynthesis RNA, Messenger/genetics RNA, Viral/genetics Simplexvirus/genetics Transcription, Genetic Viral Proteins/genetics
Chemicals
DNA, Recombinant RNA, Messenger RNA, Viral Viral Proteins DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Costa R H
Devi B G
Anderson K P
Gaylord B H
Wagner E K
References (38)
38 references, click to expand
  1. A membrane-filter technique for the detection of complementary DNA.
    Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888
  2. Detailed characterization of the mRNA mapping in the HindIII fragment K region of the herpes simplex virus type 1 genome.
    J Virol. 1981 Mar;37(3):1011-27 PMID: 6262521
  3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  4. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 PMID: 4559594
  5. The process of infection with coliphage T7. VII. Characterization and mapping of the major in vivo transcription products of the early region.
    J Mol Biol. 1973 Mar 5;74(3):291-300 PMID: 4571232
  6. Secondary structure maps of RNA: processing of HeLa ribosomal RNA.
    Proc Natl Acad Sci U S A. 1973 Oct;70(10):2827-31 PMID: 4517935
  7. Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid.
    Biochemistry. 1974 Aug 13;13(17):3606-15 PMID: 4858491
  8. Culture medium for enterobacteria.
    J Bacteriol. 1974 Sep;119(3):736-47 PMID: 4604283
  9. Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid.
    J Bacteriol. 1975 Jan;121(1):354-62 PMID: 1090574
  10. Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.
    J Mol Biol. 1975 Jan 25;91(3):315-28 PMID: 1102702
  11. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  12. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  13. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.
    Anal Biochem. 1976 Jan;70(1):75-85 PMID: 1259158
  14. Efficeint transcription of RNA into DNA by avian sarcoma virus polymerase.
    Biochim Biophys Acta. 1976 Sep 6;442(3):324-30 PMID: 183818
  15. Quantitation of herpes simplex virus type 1 RNA in infected HeLa cells.
    J Virol. 1977 Mar;21(3):889-901 PMID: 191652
  16. Zea mays chloroplast ribosomal RNA genes are part of a 22,000 base pair inverted repeat.
    Cell. 1977 Aug;11(4):739-49 PMID: 890739
  17. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  18. Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation.
    Proc Natl Acad Sci U S A. 1977 Oct;74(10):4370-4 PMID: 270678
  19. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8 PMID: 73185
  20. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350-4 PMID: 414220
  21. Spliced early mRNAs of simian virus 40.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1274-8 PMID: 206891
  22. Anatomy of herpes simplex virus (HSV) DNA. X. Mapping of viral genes by analysis of polypeptides and functions specified by HSV-1 X HSV-2 recombinants.
    J Virol. 1978 May;26(2):389-410 PMID: 207894
  23. Mapping early transcripts of herpes simplex virus type 1 by electron microscopy.
    J Virol. 1978 Jul;27(1):56-73 PMID: 211255
  24. Physical mapping of herpes simplex virus-induced polypeptides.
    J Virol. 1978 Nov;28(2):624-42 PMID: 214583
  25. Cloning of herpes simplex type 1 DNA fragments in a bacteriophage lambda vector.
    Science. 1979 Feb 9;203(4380):541-4 PMID: 216076
  26. The structure and isomerization of herpes simplex virus genomes.
    Cell. 1979 Mar;16(3):481-94 PMID: 222462
  27. Isolation and localization of herpes simplex virus type 1 mRNA.
    J Virol. 1979 Jun;30(3):805-20 PMID: 225543
  28. Isolation and localization of herpes simplex virus type 1 mRNA abundant before viral DNA synthesis.
    J Virol. 1979 Aug;31(2):447-62 PMID: 225564
  29. Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3755-9 PMID: 226978
  30. Separation and characterization of herpes simplex virus type 1 immediate-early mRNA's.
    J Virol. 1979 Jul;31(1):42-52 PMID: 228058
  31. BamI, KpnI, and SalI restriction enzyme maps of the DNAs of herpes simplex virus strains Justin and F: occurrence of heterogeneities in defined regions of the viral DNA.
    J Virol. 1979 Nov;32(2):429-41 PMID: 228068
  32. Adenovirus proteins and their messenger RNAs.
    Adv Virus Res. 1979;25:357-405 PMID: 393099
  33. Viral DNA synthesis is required for the efficient expression of specific herpes simplex virus type 1 mRNA species.
    Virology. 1980 Feb;101(1):10-24 PMID: 6243816
  34. The gene for polypeptide IX of adenovirus type 2 and its unspliced messenger RNA.
    Cell. 1980 Mar;19(3):671-81 PMID: 6985473
  35. Characterization of herpes simplex virus type 1 RNA present in the absence of de novo protein synthesis.
    J Virol. 1980 Apr;34(1):9-27 PMID: 6246265
  36. Isolation and translation of mRNA encoded by a specific region of the herpes simplex virus type 1 genome.
    J Virol. 1980 Feb;33(2):749-59 PMID: 6251246
  37. Structures of two spliced herpes simplex virus type 1 immediate-early mRNA's which map at the junctions of the unique and reiterated regions of the virus DNA S component.
    J Virol. 1981 Jan;37(1):431-44 PMID: 6260993
  38. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.38.2.483-496.1981
Published
1981-05-00
Pages
483-96
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC171179
Subset
IM
Grants
NIAAA NIH HHS · AA03506 · United States
NCI NIH HHS · CA11861 · United States
External Links
PubMed source DOI: 10.1128/JVI.38.2.483-496.1981 PMC Full Text View journal details
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