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PMID: 6287422 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of a chromosomal rearranged lambda 2 immunoglobulin gene of mouse.

Nucleic acids research ·Vol. 10 ·No. 13 ·1982-07-10 ·Pages 3831-43

Wu GE, Govindji N, Hozumi N, Murialdo H

Abstract

The rearranged lambda 2 gene of the mouse plasmacytoma cell line MOPC315 has been cloned and sequenced. A comparison of its sequence with the sequence of the unrearranged (germ-line) V, J and C gene segments shows that the sequences of the V gene segments differ at six positions. The sequence of the J and C gene segments remained unchanged. These results add support to the hypothesis that somatic mutations occur in immunoglobulin in genes and that these mutations do not involve the C gene segment. The degree of homology of the elements of the lambda 2 gene with those of the lambda 1 gene and C lambda 3 and C lambda 4 gene fragments suggest a pathway of evolution by gene duplication of the immunoglobulin lambda light chain locus. According to this scheme the original structure V0-J0C0 gave rise to a structure V0-J1C1-J11C11 by duplication of the J0C0 region. A second duplication encompassing the whole region resulted in the present structure: V1-J3C3-J1C1/V2-J2C2-J4C4.

MeSH Terms
Animals Base Sequence Cell Line Chromosomes/analysis Cloning, Molecular DNA/metabolism DNA Restriction Enzymes Genes Immunoglobulin Light Chains/genetics Immunoglobulin lambda-Chains/genetics Mice Nucleic Acid Hybridization Plasmacytoma Plasmids
Chemicals
Immunoglobulin Light Chains Immunoglobulin lambda-Chains DNA DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wu G E
Govindji N
Hozumi N
Murialdo H
References (32)
32 references, click to expand
  1. Variability in the lambda light chain sequences of mouse antibody.
    Nature. 1970 Dec 12;228(5276):1045-7 PMID: 5483159
  2. Selection under a somatic model.
    Cell Immunol. 1970 Nov;1(5):461-7 PMID: 4108695
  3. Amino acid sequence of the light chain of a mouse myeloma protein (MOPC-315).
    Biochemistry. 1973 Dec 18;12(26):5400-16 PMID: 4760498
  4. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  5. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  6. Sequence of a mouse germ-line gene for a variable region of an immunoglobulin light chain.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1485-9 PMID: 418414
  7. A complete immunoglobulin gene is created by somatic recombination.
    Cell. 1978 Sep;15(1):1-14 PMID: 100225
  8. Sequences of mouse immunoglobulin light chain genes before and after somatic changes.
    Cell. 1978 Dec;15(4):1133-44 PMID: 103630
  9. Domains and the hinge region of an immunoglobulin heavy chain are encoded in separate DNA segments.
    Nature. 1979 Feb 22;277(5698):627-33 PMID: 106304
  10. Sequences of five potential recombination sites encoded close to an immunoglobulin kappa constant region gene.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3450-4 PMID: 115000
  11. The joining of V and J gene segments creates antibody diversity.
    Nature. 1980 Jan 31;283(5746):497-9 PMID: 6766210
  12. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  13. An immunoglobulin heavy chain variable region gene is generated from three segments of DNA: VH, D and JH.
    Cell. 1980 Apr;19(4):981-92 PMID: 6769593
  14. Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genes.
    Nature. 1980 Aug 14;286(5774):676-83 PMID: 6774258
  15. Nucleotide sequence of immunoglobulin heavy chain joining segments between translocated VH and mu constant regions genes.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3630-4 PMID: 6251474
  16. Variation in the crossover point of kappa immunoglobulin gene V-J recombination: evidence from a cryptic gene.
    Cell. 1980 Oct;21(3):793-9 PMID: 6777049
  17. Dual expression of lambda genes in the MOPC-315 plasmacytoma.
    Nature. 1981 Mar 5;290(5801):65-7 PMID: 6259534
  18. The nucleotide sequence of a 5.5-kilobase DNA segment containing the mouse kappa immunoglobulin J and C region genes.
    J Biol Chem. 1981 May 25;256(10):5116-20 PMID: 6262318
  19. IgG antibodies to phosphorylcholine exhibit more diversity than their IgM counterparts.
    Nature. 1981 May 7;291(5810):29-34 PMID: 7231520
  20. DNA sequence of the constant gene region of the mouse immunoglobulin kappa chain.
    Nucleic Acids Res. 1981 Feb 25;9(4):971-81 PMID: 6785724
  21. Molecular cloning of an immunoglobulin kappa constant gene from NZB mouse.
    Gene. 1981 Mar;13(2):163-172 PMID: 6453743
  22. Identification of a third type of lambda light chain in mouse immunoglobulins.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):569-73 PMID: 6165998
  23. Organization of four mouse lambda light chain immunoglobulin genes.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3765-9 PMID: 6791160
  24. Somatic mutation of immunoglobulin light-chain variable-region genes.
    Cell. 1981 Jul;25(1):47-58 PMID: 6791832
  25. The nucleotide sequences of rearranged and germline immunoglobulin VH genes of a mouse myeloma MC101 and evolution of VH genes in mouse.
    J Biol Chem. 1982 Jan 10;257(1):277-85 PMID: 6273429
  26. RNA splicing mutation in an aberrantly rearranged immunoglobulin lambda I gene.
    Proc Natl Acad Sci U S A. 1981 Nov;78(11):7019-23 PMID: 6171827
  27. Structural alterations in J regions of mouse immunoglobulin lambda genes are associated with differential gene expression.
    Nature. 1982 Feb 4;295(5848):428-30 PMID: 6799835
  28. Somatic diversification is required to generate the V kappa genes of MOPC 511 and MOPC 167 myeloma proteins.
    Proc Natl Acad Sci U S A. 1981 Dec;78(12):7674-8 PMID: 6801657
  29. Complete amino acid sequence of light chain variable regions derived from five monoclonal anti-p-azophenylarsonate antibodies differing with respect to a crossreactive idiotype.
    Proc Natl Acad Sci U S A. 1981 Dec;78(12):7679-83 PMID: 6801658
  30. DNA sequences of the joining regions of mouse lambda light chain immunoglobulin genes.
    Proc Natl Acad Sci U S A. 1982 Jan;79(2):530-3 PMID: 6804942
  31. Antibody diversity: somatic hypermutation of rearranged VH genes.
    Cell. 1981 Dec;27(3 Pt 2):573-81 PMID: 6101208
  32. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.13.3831
Published
1982-07-10
Pages
3831-43
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320762
Subset
IM
Databases
GENBANK
J00591, J00592
External Links
PubMed source DOI: 10.1093/nar/10.13.3831 PMC Full Text View journal details
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