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PMID: 6286719 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification and typing of herpes simplex viruses with monoclonal antibodies.

Journal of clinical microbiology ·Vol. 16 ·No. 1 ·1982-07-00 ·Pages 205-8

Balachandran N, Frame B, Chernesky M, Kraiselburd E, Kouri Y, Garcia D, Lavery C, Rawls WE

Abstract

Monoclonal antibodies which reacted with type-specific antigens of herpes simplex virus type 2 or with antigens shared by herpes simplex virus types 1 and 2 were used in an indirect immunofluorescence assay to type virus isolates and to detect viral antigens in cells obtained from herpetic lesions. Complete concordance was obtained for 42 isolates typed by endonuclease restriction analysis of viral DNA and by indirect immunofluorescence with monoclonal antibodies. Examination of a limited number of ulcerative lesions revealed that indirect immunofluorescence and virus isolation were comparable in detecting herpes simplex virus. The results indicate that monoclonal antibodies can be used to accurately identify and type isolates of herpes simplex virus.

MeSH Terms
Animals Antibodies, Monoclonal Antigens, Viral/analysis Herpes Simplex/microbiology Humans Hybridomas/immunology Mice Simplexvirus/classification,immunology
Chemicals
Antibodies, Monoclonal Antigens, Viral
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Balachandran N
Frame B
Chernesky M
Kraiselburd E
Kouri Y
Garcia D
Lavery C
Rawls W E
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32 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.16.1.205-208.1982
Published
1982-07-00
Pages
205-8
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC272324
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.16.1.205-208.1982 PMC Full Text View journal details
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