Rapid typing of herpes simplex virus strains using the indirect immunoperoxidase method.

Applied microbiology ·Vol. 28 ·No. 4 ·1974-10-00 ·Pages 568-71

Benjamin DR

Abstract

The indirect immunoperoxidase technique with type-specific antisera was compared to kinetic neutralization for the typing of herpes simplex virus strains. There was complete agreement between the results obtained by kinetic neutralization and indirect immunoperoxidase. The indirect immunoperoxidase assay was far simpler to perform and interpret than the kinetic neutralization tests, and it offered a rapid means for the routine antigenic typing of herpes simplex isolates.

MeSH Terms

Animals Antigens, Viral/analysis Encephalitis/cerebrospinal fluid Evaluation Studies as Topic Guinea Pigs/immunology Herpes Simplex/cerebrospinal fluid Humans Immune Sera Neutralization Tests Peroxidases/immunology Rabbits/immunology Serotyping/methods Simplexvirus/classification Time Factors

Chemicals

Antigens, Viral Immune Sera Peroxidases

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Benjamin D R

References (15)

15 references, click to expand

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Type-specific surface antigens of cells infected with herpes simplex virus (1 and 2).
Proc Soc Exp Biol Med. 1971 Oct;138(1):21-7 PMID: 4331091
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Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration.
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Proc Soc Exp Biol Med. 1974 Jun;146(2):432-7 PMID: 4365311
Use of immunoperoxidase for the rapid identification of human myxoviruses and paramyxoviruses in tissue culture.
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SIMPLIFIED MYELOPEROXIDASE STAIN USING BENZIDINE DIHYDROCHLORIDE.
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Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.28.4.568-571.1974

Published

1974-10-00

Pages

568-71

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC186773

Subset

External Links

PubMed source DOI: 10.1128/am.28.4.568-571.1974 PMC Full Text

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Rapid typing of herpes simplex virus strains using the indirect immunoperoxidase method.

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