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PMID: 6285288 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular cloning and analysis of yeast gene for cycloheximide resistance and ribosomal protein L29.

Nucleic acids research ·Vol. 10 ·No. 10 ·1982-05-25 ·Pages 3133-48

Fried HM, Warner JR

Abstract

A cosmid clone bank of yeast DNA has been used to isolate the cycloheximide resistance gene cyh2 of Saccharomyces cerevisiae. A cosmid carrying this gene was identified by cross hybridization to another cloned gene, tsm437. The two genes, which are tightly linked genetically are both present on a 31 kb segment of cloned DNA. The cyh2 gene encodes ribosomal protein L29, a component of the large subunit. Blot hybridization analysis reveals that this gene is present as a single copy in the yeast genome, unlike many other yeast ribosomal protein genes which appear to be duplicated. The cyh2 gene also appears to contain an intervening sequence, a characteristic common to most yeast ribosomal protein genes that have been cloned.

MeSH Terms
Alleles Cloning, Molecular Cycloheximide/pharmacology DNA Restriction Enzymes Drug Resistance, Microbial Genes Nucleic Acid Hybridization Plasmids Protein Biosynthesis Ribosomal Proteins/genetics Saccharomyces cerevisiae/drug effects,genetics,growth & development
Chemicals
Ribosomal Proteins ribosomal protein L29 Cycloheximide DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fried H M
Warner J R
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30 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.10.3133
Published
1982-05-25
Pages
3133-48
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320696
Subset
IM
Grants
NCI NIH HHS · CA 13330 · United States
NIGMS NIH HHS · GM 07165 · United States
NIGMS NIH HHS · GM 25532 · United States
External Links
PubMed source DOI: 10.1093/nar/10.10.3133 PMC Full Text View journal details
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