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PMID: 6285143 Published · ppublish English Journal Article

Influence of phage T3 and T7 gene functions on a type III(EcoP1) DNA restriction-modification system in vivo.

Molecular & general genetics : MGG ·Vol. 185 ·No. 3 ·1982-00-00 ·Pages 457-61

Krüger DH, Reuter M, Hansen S, Schroeder C

Abstract

The ocr+ gene function (gp 0.3) of bacteriophages T3 and T7 not only counteracts type I (EcoB, EcoK) but also type III restriction endonucleases (EcoP1). Despite the presence of recognition sites, phage DNA as well as simultaneously introduced plasmid DNA are protected by ocr+ expression against both the endonucleolytic and the methylating activities of the EcoP1 enzyme. Nevertheless, the EcoP1 protein causes the exclusion of T3 and T7 in P1-lysogenic cells, apparently by exerting a repressor-like effect on phage gene expression. T3 which induces an S-adenosylmethionine hydrolase is less susceptible to the repressor effect of the SAM-stimulated EcoP1 enzyme. The abundance of EcoP1 recognition sites in the T7 genome is explained by their near identity with the T7 DNA primase recognition site.

MeSH Terms
DNA Replication DNA Restriction Enzymes DNA, Bacterial/genetics DNA, Viral/genetics Escherichia coli/genetics Genes, Viral Kinetics Mutation T-Phages/genetics Transformation, Bacterial Virus Replication
Chemicals
DNA, Bacterial DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Krüger D H
Reuter M
Hansen S
Schroeder C
References (33)
33 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00334140
Published
1982-00-00
Pages
457-61
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00334140
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