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PMID: 6283471 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Cloning and characterization of cDNA copies of the 7S RNAs of HeLa cells.

Nucleic acids research ·Vol. 10 ·No. 7 ·1982-04-10 ·Pages 2209-23

Ullu E, Melli M

Abstract

We have cloned cDNA copies of in vitro adenylated 7S RNA of HeLa cells. The most representative clones in the library contain DNA fragments copied from the 7SL and 7SK small RNAs. The two classes of recombinants share no homology. The 7SL RNA contains at the 5' end of the molecule sequences homologous to the Alu sequence family. Hybridization to human genomic DNA shows that the 7SL and 7SK clones are homologous to two different families of repetitive sequences.

MeSH Terms
Cloning, Molecular DNA DNA Restriction Enzymes DNA, Recombinant/metabolism HeLa Cells/metabolism Humans Molecular Weight Nucleic Acid Hybridization RNA, Neoplasm/genetics Repetitive Sequences, Nucleic Acid
Chemicals
DNA, Recombinant RNA, Neoplasm DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ullu E
Melli M
References (28)
28 references, click to expand
  1. On the lability of poly(A) sequences during extraction of messenger RNA from polyribosomes.
    Biochim Biophys Acta. 1972 Mar 14;262(2):220-6 PMID: 5017913
  2. Comparison of oligonucleotides produced by RNase T1 digestion of 7 S RNA from avian and murine oncornaviruses and from uninfected cells.
    Virology. 1973 May;53(1):40-6 PMID: 4350418
  3. Purification and characterization of adenosine triphosphate: ribonucleic acid adenyltransferase from Escherichia coli.
    Eur J Biochem. 1973 Aug 1;37(1):31-40 PMID: 4580885
  4. The 7S RNA common to oncornaviruses and normal cells is associated with polyribosomes.
    Proc Natl Acad Sci U S A. 1974 Sep;71(9):3390-4 PMID: 4530311
  5. Clustering of the DNA sequences complementary to repetitive nuclear RNA of HeLa cells.
    J Mol Biol. 1975 Mar 25;93(1):23-38 PMID: 1152047
  6. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.
    Biochemistry. 1975 Aug 26;14(17):3787-94 PMID: 1174504
  7. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  8. Small RNA species of the HeLa cell: metabolism and subcellular localization.
    Cell. 1976 May;8(1):19-31 PMID: 954090
  9. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  10. Synthesis of double-stranded DNA complementary to lysozyme, ovomucoid, and ovalbumin mRNAs. Optimization for full length second strand synthesis by Escherichia coli DNA polymerase I.
    J Biol Chem. 1978 Apr 10;253(7):2483-95 PMID: 75887
  11. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  12. Retroviruses.
    Annu Rev Biochem. 1978;47:35-88 PMID: 354499
  13. 3'-terminal labelling of RNA with T4 RNA ligase.
    Nature. 1978 Oct 12;275(5680):560-1 PMID: 692735
  14. The isolation of structural genes from libraries of eucaryotic DNA.
    Cell. 1978 Oct;15(2):687-701 PMID: 719759
  15. A ubiquitous family of repeated DNA sequences in the human genome.
    J Mol Biol. 1979 Aug 15;132(3):289-306 PMID: 533893
  16. Partial nucleotide sequence of the 300-nucleotide interspersed repeated human DNA sequences.
    Nature. 1980 Mar 27;284(5754):372-4 PMID: 6244506
  17. An abundant cytoplasmic 7S RNA is complementary to the dominant interspersed middle repetitive DNA sequence family in the human genome.
    Cell. 1980 Nov;22(1 Pt 1):209-18 PMID: 6159101
  18. Intracellular distribution of low molecular weight RNA species in HeLa cells.
    J Cell Biol. 1980 NOV;87(2 Pt 1):398-403 PMID: 6159360
  19. Nucleotide sequences of 4.5S RNAs associated with poly(A)-containing RNAs of mouse and hamster cells.
    Nucleic Acids Res. 1980 Mar 25;8(6):1273-85 PMID: 6159592
  20. Structural analysis of interspersed repetitive polymerase III transcription units in human DNA.
    Nucleic Acids Res. 1981 Mar 11;9(5):1151-70 PMID: 7232213
  21. Transcriptional analysis of interspersed repetitive polymerase III transcription units in human DNA.
    Nucleic Acids Res. 1981 Mar 11;9(5):1171-89 PMID: 7232214
  22. Structural analysis of templates and RNA polymerase III transcripts of Alu family sequences interspersed among the human beta-like globin genes.
    Gene. 1981 Mar;13(2):185-96 PMID: 6165649
  23. Differential and coordinate regulation of the eukaryotic small molecular weight RNAs.
    J Biol Chem. 1981 Jul 10;256(13):6663-9 PMID: 7240236
  24. Characterization of the association of two small molecular weight RNAs with eukaryotic polysomes.
    J Biol Chem. 1981 Jul 10;256(13):6670-5 PMID: 6165722
  25. Characterization and subcellular localization of 7-8 S RNAs of Novikoff hepatoma.
    J Biol Chem. 1981 Aug 25;256(16):8452-7 PMID: 6167578
  26. Two groups of small stable RNAs.
    Cell. 1981 Aug;25(2):296-7 PMID: 6169437
  27. Isolation of cloned genes differentially expressed at early and late stages of Drosophila embryonic development.
    Dev Biol. 1981 Sep;86(2):438-47 PMID: 6269930
  28. Base sequence studies of 300 nucleotide renatured repeated human DNA clones.
    J Mol Biol. 1981 Sep 5;151(1):17-33 PMID: 6276559
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.7.2209
Published
1982-04-10
Pages
2209-23
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320604
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.7.2209 PMC Full Text View journal details
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