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PMID: 6281237 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Construction from Mu d1 (lac Apr) lysogens of lambda bacteriophage bearing promoter-lac fusions: isolation of lambda ppheA-lac.

Journal of bacteriology ·Vol. 150 ·No. 3 ·1982-06-00 ·Pages 1122-9

Gowrishankar J, Pittard J

Abstract

Bacteriophage Mu d1 (lac Aprr) was used to obtain strains of Escherichia coli K-12 in which the lac genes are expressed from the promoter of pheA, the structural gene for the enzyme chorismate mutase P-prephenate-dehydratase. A derivative of bacteriophage lambda which carries the pheA-lac fusion was prepared; the method used is generally applicable for the construction, from Mu dl lysogens, of specialized transducing lambda phage carrying the promoter-lac fusions. A restriction enzyme cleavage map of lambda ppheA-lac for the enzymes HindIII and PstI is presented.

MeSH Terms
Bacteriophage lambda/genetics Bacteriophage mu/genetics Base Sequence DNA Restriction Enzymes DNA, Recombinant Deoxyribonuclease HindIII Deoxyribonucleases, Type II Site-Specific Genes Hydro-Lyases/genetics Lac Operon Lysogeny Operon Prephenate Dehydratase/genetics Transduction, Genetic
Chemicals
DNA, Recombinant DNA Restriction Enzymes Deoxyribonuclease HindIII CTGCAG-specific type II deoxyribonucleases Deoxyribonucleases, Type II Site-Specific Hydro-Lyases Prephenate Dehydratase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gowrishankar J
Pittard J
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25 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.150.3.1122-1129.1982
Published
1982-06-00
Pages
1122-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216332
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.150.3.1122-1129.1982 PMC Full Text View journal details
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