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PMID: 6280816 Published · ppublish English Comparative Study Journal Article

Nontumoral, benign ad malignant stages of transformation of a diploid pig cell line. A review.

Canadian journal of comparative medicine : Revue canadienne de medecine comparee ·Vol. 45 ·No. 3 ·1981-07-00 ·Pages 279-90

Bouillant AM, Genest P, Greig AS

Abstract

The PFT cell line originated from diploid endometrial cells and was subcultured more than 500 times. Nontumoral, benign and malignant transformation appeared at different stages. Four populations (F1, F2, F3 and F4) of cells were demonstrated sequentially by chromosomes analysis. These consisted of eight phenotypes corresponding to the sequential appearance of markers which included three chromosomal aberrations. The PFT malignant transformation in vitro is a progressive process through qualitatively different stages and may reflect neoplastic development in vivo. It is suggested that the benign and malignant transformations were under separate genetic control since they occurred after two chromosomal translocations on two different chromosomes. The spontaneous expression of a pig endogenous type-C virus which occurred concomitantly with the first translocation appeared to be related to oncogenesis.

MeSH Terms
Animals Cell Line Cell Transformation, Neoplastic/pathology,ultrastructure Concanavalin A/pharmacology Culture Media Diploidy Endometrium Female Hydrogen-Ion Concentration Karyotyping Mice Mice, Nude Microscopy, Electron Neoplasms, Experimental/etiology Phenotype Retroviridae/growth & development Swine Translocation, Genetic Virus Replication
Chemicals
Culture Media Concanavalin A
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bouillant A M
Genest P
Greig A S
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Article Info
Journal
Canadian journal of comparative medicine : Revue canadienne de medecine comparee
Abbr.
Can J Comp Med
ISSN
0008-4050
Published
1981-07-00
Pages
279-90
Language
English
Region
Canada
NLM ID
0151747
PMCID
PMC1320221
Subset
IM
External Links
PubMed source PMC Full Text
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