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PMID: 6278431 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The sequence of the nucleoprotein gene of human influenza A virus, strain A/NT/60/68.

Nucleic acids research ·Vol. 10 ·No. 3 ·1982-02-11 ·Pages 1029-38

Huddleston JA, Brownlee GG

Abstract

The nucleotide sequence of the nucleoprotein gene of influenza A/NT/60/68 was established after using improved cloning methods to obtain full length cDNA clones in pBr322. The gene is 1565 residues long and codes for a basic protein of 498 amino acids. There are only 30 amino acid differences between it and the homologous sequence in A/PR/8/35, all occurring as point mutations. Assuming a common lineage, the evolutionary rate of divergence of the two strains is 0.18% amino acid per year. This confirms there is a slow but significant rate of evolution.

MeSH Terms
Amino Acid Sequence Base Sequence Biological Evolution Cloning, Molecular DNA DNA Restriction Enzymes Deoxyribonucleoproteins Genes, Viral Humans Influenza A virus/genetics Mutation Nucleoproteins Orthomyxoviridae/genetics Plasmids Viral Proteins/genetics
Chemicals
Deoxyribonucleoproteins Nucleoproteins Viral Proteins DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Huddleston J A
Brownlee G G
References (23)
23 references, click to expand
  1. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  2. Phosphorylated protein component present in influenza virions.
    J Virol. 1977 Oct;24(1):401-5 PMID: 904030
  3. Nucleotide sequences at the 5' termini of influenza virus RNAs and their transcripts.
    Nucleic Acids Res. 1978 Apr;5(4):1207-19 PMID: 652519
  4. The genome of the influenza virus.
    Curr Top Microbiol Immunol. 1978;80:139-69 PMID: 668407
  5. The influenza virus genome and its replication.
    Br Med Bull. 1979 Jan;35(1):39-46 PMID: 367485
  6. A new method for the size estimation of the RNA genome segments of influenza virus.
    Nucleic Acids Res. 1979 Apr;6(4):1309-21 PMID: 88039
  7. Evidence for antigenic variation in influenza A nucleoprotein.
    Virology. 1979 Mar;93(2):569-73 PMID: 88124
  8. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  9. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  10. Categorization of nucleoproteins and matrix proteins from type A influenza viruses by peptide mapping.
    Virology. 1980 Jun;103(2):350-6 PMID: 7385584
  11. Sequence of interrupted and uninterrupted mRNAs and cloned DNA coding for the two overlapping nonstructural proteins of influenza virus.
    Cell. 1980 Sep;21(2):475-85 PMID: 7407920
  12. Antigenic variation of influenza A virus nucleoprotein detected with monoclonal antibodies.
    J Virol. 1980 Jul;35(1):24-30 PMID: 6157838
  13. A new computer method for the storage and manipulation of DNA gel reading data.
    Nucleic Acids Res. 1980 Aug 25;8(16):3673-94 PMID: 7433103
  14. Cloning of influenza cDNA ino M13: the sequence of the RNA segment encoding the A/PR/8/34 matrix protein.
    Nucleic Acids Res. 1980 May 10;8(9):1965-74 PMID: 6927841
  15. Two forms of influenza virus nucleoprotein in infected cells and virions.
    Virology. 1981 Feb;109(1):174-9 PMID: 7467131
  16. The use of synthetic oligodeoxynucleotide primers in cloning and sequencing segment of 8 influenza virus (A/PR/8/34).
    Nucleic Acids Res. 1981 Jan 24;9(2):237-45 PMID: 7208353
  17. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
  18. Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route.
    Nucleic Acids Res. 1981 Apr 10;9(7):1691-706 PMID: 7232224
  19. Complete nucleotide sequence of the nucleoprotein gene from the human influenza strain A/PR/8/34 (HON1).
    Eur J Biochem. 1981 May 15;116(2):347-53 PMID: 6166474
  20. Electrophoretic separation of influenza virus ribonucleoproteins.
    J Gen Virol. 1981 Mar;53(Pt 1):125-32 PMID: 7276909
  21. Nucleotide sequence of the haemagglutinin gene of a human influenza virus H1 subtype.
    Nature. 1981 Jul 2;292(5818):72-5 PMID: 7278968
  22. Sequences of mRNAs derived from genome RNA segment 7 of influenza virus: colinear and interrupted mRNAs code for overlapping proteins.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4170-4 PMID: 6945577
  23. The structure of the gene encoding the nucleoprotein of human influenza virus A/PR/8/34.
    Virology. 1981 Oct 30;114(2):423-8 PMID: 7292985
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.3.1029
Published
1982-02-11
Pages
1029-38
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326218
Subset
IM
Databases
GENBANK
J02137
External Links
PubMed source DOI: 10.1093/nar/10.3.1029 PMC Full Text View journal details
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