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PMID: 6278422 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Moloney murine leukemia virus-induced tumors: recombinant proviruses in active chromatin regions.

Nucleic acids research ·Vol. 10 ·No. 2 ·1982-01-22 ·Pages 577-92

van der Putten H, Quint W, Verma IM, Berns A

Abstract

The DNase I sensitivity of chromosomal DNA regions carrying integrated proviral genomes of Moloney (M-MuLV) and AKR Murine Leukemia Virus (AKR-MuLV), and the cellular homologue of the mos-gene (c-mos) of Moloney Sarcoma Virus (MSV) were studied in tumor tissues of leukemic mice. The genetically transmitted sequences of M-MuLV, AKR-MuLV, and the c-mos gene are all in DNase I resistant chromatin conformations in M-MuLV-induced tumors. Each M-MuLV-induced tumor contained at least one somatically acquired integrated recombinant MuLV genome that displayed two main characteristic features of active chromatin: a) a configuration hypersensitive to DNase I, and b) extensive hypomethylation. DNase I hypersensitive sites were mapped at the junction of cellular sequences and the 5'-viral large terminal repeat (LTR). Expression of a recombinant MuLV seems therefore to be a necessary feature to maintain the transformed state.

MeSH Terms
AKR murine leukemia virus/genetics Animals Cell Line Cell Nucleus/physiology Cell Transformation, Neoplastic Chromatin/physiology DNA Restriction Enzymes DNA, Recombinant/analysis Deoxyribonuclease I Deoxyribonucleases Endonucleases Leukemia, Experimental/microbiology Methylation Mice Mice, Inbred BALB C Moloney murine leukemia virus/genetics
Chemicals
Chromatin DNA, Recombinant Deoxyribonucleases Endonucleases DNA Restriction Enzymes Deoxyribonuclease I
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
van der Putten H
Quint W
Verma I M
Berns A
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27 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.2.577
Published
1982-01-22
Pages
577-92
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326159
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.2.577 PMC Full Text View journal details
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