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PMID: 6277716 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cyclic AMP: a potent inhibitor of DNA synthesis in cultured arterial endothelial and smooth muscle cells.

Diabetologia ·Vol. 22 ·No. 1 ·1982-01-00 ·Pages 51-5

Stout RW

Abstract

The effect of dibutyryl cyclic AMP on DNA synthesis was studied in cultured human umbilical endothelial cells and rat aortic smooth muscle cells. Dibutyryl cyclic AMP (2 X 10(-4) mol/l) inhibited DNA synthesis in both arterial cell types when they were grown in medium supplemented with whole serum or with platelet poor serum, but had no effect in the absence of serum. An effect was seen one hour after the addition of the nucleotide, and the threshold concentration was between 2 X 10(-6) and 2 X 10(-5) mol/l. These results may have relevance to the interaction of platelets and insulin with the arterial wall in the development of atherosclerosis in diabetes.

MeSH Terms

Bucladesine/pharmacology Cells, Cultured Cyclic AMP/pharmacology DNA/biosynthesis Endothelium/metabolism Humans Muscle, Smooth, Vascular/metabolism Umbilical Veins/metabolism

Chemicals

Bucladesine DNA Cyclic AMP

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Stout R W

References (29)

29 references, click to expand

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Article Info

Journal

Diabetologia

Abbr.

Diabetologia

ISSN

0012-186X

DOI

10.1007/BF00253870

Published

1982-01-00

Pages

51-5

Language

English

Region

Germany

NLM ID

0006777

Subset

External Links

PubMed source DOI: 10.1007/BF00253870 View journal details

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Cyclic AMP: a potent inhibitor of DNA synthesis in cultured arterial endothelial and smooth muscle cells.

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