Home LiteratureArticle Details
PMID: 6275821 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

O-glycosidic carbohydrate-peptide linkages of Herpes simplex virus glycoproteins.

Archives of virology ·Vol. 70 ·No. 4 ·1981-00-00 ·Pages 321-9

Olofsson S, Blomberg J, Lycke E

Abstract

Electrophoretically purified HSV-specified glycoproteins with radiolabelled carbohydrates were subjected to mild alkaline borohydride treatment (0.5 M NaOH and 0.5 M NaBH4). The treatment liberated significant amounts of the labelled oligosaccharides. The latter demonstrated molecular weights of about 3,000 as determined by gel filtration. The glycoproteins involved probably belong to the gA/gB complex or gC. The results suggest that HSV specified glycoproteins contain oligosaccharides linked with an O-glycosidic bond to a threonine or serine residue of the polypeptide.

MeSH Terms
Borohydrides Chemical Phenomena Chemistry Electrophoresis, Polyacrylamide Gel Galactosamine/metabolism Glucosamine/metabolism Glycoproteins/analysis Oligosaccharides/analysis Simplexvirus/analysis,metabolism Viral Proteins/analysis
Chemicals
Borohydrides Glycoproteins Oligosaccharides Viral Proteins Galactosamine sodium borohydride Glucosamine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Olofsson S
Blomberg J
Lycke E
References (26)
26 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Comparative aspects of glycoprotein structure.
    Annu Rev Biochem. 1976;45:217-37 PMID: 786150
  3. Membrane proteins specified by herpes simplex viruses. V. Identification of an Fc-binding glycoprotein.
    J Virol. 1979 Dec;32(3):779-89 PMID: 229267
  4. Synthesis and processing of glycoproteins gD and gC of herpes simplex virus type 1.
    J Virol. 1980 Nov;36(2):429-39 PMID: 6253668
  5. Fractionation of glycopeptides by affinity column chromatography on concanavalin A-sepharose.
    J Biochem. 1975 Oct;78(4):687-96 PMID: 1213987
  6. Polyacrylamide gel electrophoretic analysis of herpes simplex virus type 1 immunoprecipitates obtained by quantitative immunoelectrophoresis in antibody-containing agarose gel.
    J Virol. 1977 Apr;22(1):113-7 PMID: 192909
  7. Structure of the complex carbohydrate chains in the membrane glycoproteins of Semliki Forest virus.
    Biochem Soc Trans. 1977;5(1):120-1 PMID: 892136
  8. Separation of Herpes simplex virus-induced antigens by Concanavalin A affinity chromatography.
    J Virol. 1973 Oct;12(4):766-74 PMID: 4359952
  9. GROWTH AND CYTOPATHIC EFFECT OF RUBELLA VIRUS IN A LINE OF GREEN MONKEY KIDNEY CELLS.
    Proc Soc Exp Biol Med. 1965 Jan;118:85-90 PMID: 14254593
  10. The role of polyprenol-linked sugars in glycoprotein synthesis.
    Annu Rev Biochem. 1976;45:95-112 PMID: 786163
  11. Studies on glycopeptides of Herpes simplex virus infected cells.
    Arch Virol. 1977;55(4):293-304 PMID: 202227
  12. Proteins specified by herpes simplex virus. XIII. Glycosylation of viral polypeptides.
    J Virol. 1975 Nov;16(5):1308-26 PMID: 171454
  13. Quantitation of oligosaccharides released by the beta-elimination reaction.
    Anal Biochem. 1980 Jan 1;101(1):44-53 PMID: 7356136
  14. Unusual lectin-binding properties of a herpes simplex virus type 1-specific glycoprotein.
    J Virol. 1981 May;38(2):564-70 PMID: 6264128
  15. Effect of tunicamycin on herpes simplex virus glycoproteins and infectious virus production.
    J Virol. 1980 Apr;34(1):142-53 PMID: 6246250
  16. Structures of O-glycosidically linked carbohydrate units of herring egg sialoglycoprotein.
    J Biochem. 1981 Apr;89(4):1067-74 PMID: 7195901
  17. Metabolism of galactose in herpes simplex virus-infected cells.
    Virology. 1976 Jan;69(1):216-28 PMID: 174290
  18. Production of herpes simplex antisera with increased type specificity in guinea pigs by antibody suppression.
    Proc Soc Exp Biol Med. 1974 Dec;147(3):788-94 PMID: 4374703
  19. Biogenesis of vaccinia: carbohydrate of the hemagglutinin molecules.
    Virology. 1981 May;111(1):56-72 PMID: 7233832
  20. Carbohydrates of influenza virus. III. Nature of oligosaccharide-protein linkage in viral glycoproteins.
    J Virol. 1979 Jul;31(1):253-6 PMID: 501797
  21. Membrane proteins specified by herpes simplex viruses. I. Identification of four glycoprotein precursors and their products in type 1-infected cells.
    J Virol. 1976 Mar;17(3):991-1008 PMID: 176453
  22. Oligosaccharide moieties of the glycoprotein of vesicular stomatitis virus.
    J Virol. 1976 Apr;18(1):167-75 PMID: 176458
  23. The poly(glycosyl) chains of glycoproteins. Characterisation of a novel type of glycoprotein saccharides from human erythrocyte membrane.
    Eur J Biochem. 1978 Dec 1;92(1):289-300 PMID: 729592
  24. gA and gB glycoproteins of herpes simplex virus type 1: two forms of a single polypeptide.
    J Virol. 1980 Dec;36(3):665-75 PMID: 6162034
  25. Protein-carbohydrate interaction. 13. The interaction of concanavalin A with alpha-mannans from a variety of microorganisms.
    J Biol Chem. 1968 Apr 25;243(8):2003-7 PMID: 4967174
  26. Two-dimensional gel analysis of HSV type 1-induced polypeptides and glycoprotein processing.
    J Gen Virol. 1981 Jan;52(Pt 1):77-92 PMID: 6267177
Article Info
Journal
Archives of virology
Abbr.
Arch Virol
ISSN
0304-8608
DOI
10.1007/BF01320247
Published
1981-00-00
Pages
321-9
Language
English
Region
Austria
NLM ID
7506870
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01320247 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2