Home LiteratureArticle Details
PMID: 6275363 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

5S ribosomal RNA genes of the newt Notophthalmus viridescens.

Nucleic acids research ·Vol. 9 ·No. 23 ·1981-12-11 ·Pages 6457-69

Kay BK, Gall JG

Abstract

The genes which code for the 5S ribosomal RNA in the newt, Notophthalmus viridescens have been cloned and analyzed. Two types of repeating unit were detected: a major type consisting of a 120 bp coding region with a 111 bp spacer, and a minor type composed of a coding region, a pseudogene, and a 113 bp spacer. The pseudogene is a 36 bp segment which corresponds to the 3' terminal third of the 5S RNA gene, and is situated immediately 3' to the gene, being separated from it by 2 bp. Two recombinant plasmids were obtained in which the major and minor units were arranged in an interspersed pattern.

MeSH Terms
Animals Base Composition Base Sequence Cloning, Molecular DNA Restriction Enzymes Genes Molecular Sequence Data Nucleic Acid Hybridization RNA, Ribosomal/genetics Salamandridae
Chemicals
RNA, Ribosomal DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kay B K
Gall J G
References (25)
25 references, click to expand
  1. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  2. Repeated genes in eukaryotes.
    Annu Rev Biochem. 1980;49:727-64 PMID: 6996571
  3. Sequence and heterogeneity in the 5S RNA gene cluster of Drosophila melanogaster.
    Nucleic Acids Res. 1980 Feb 11;8(3):441-51 PMID: 6160476
  4. In vitro RNA synthesis in oocyte nuclei of the newt Notophthalmus.
    Chromosoma. 1981;82(2):171-87 PMID: 6164523
  5. In vitro transcription of cloned 5S RNA genes of the newt Notophthalmus.
    J Cell Biol. 1981 Aug;90(2):323-31 PMID: 6945306
  6. The nucleotide sequence of oocyte 5S DNA in Xenopus laevis. II. The GC-rich region.
    Cell. 1978 Apr;13(4):717-25 PMID: 566164
  7. Specific gene amplification in oocytes. Oocyte nuclei contain extrachromosomal replicas of the genes for ribosomal RNA.
    Science. 1968 Apr 19;160(3825):272-80 PMID: 4867987
  8. Gene linkage by RNA-DNA hybridization. I. Unique DNA sequences homologous to 4 s RNA, 5 s RNA and ribosomal RNA.
    J Mol Biol. 1968 Jun 28;34(3):661-80 PMID: 5760467
  9. Visualization of RNA synthesis on chromosomes.
    Int Rev Cytol. 1972;33:1-25 PMID: 4562602
  10. The mitotic chromosomes of Notophthalmus (=Triturus) viridescens: localization of C banding regions and DNA sequences complementary to 18S, 28S and 5S ribosomal RNA.
    Chromosoma. 1975 Nov 20;53(1):51-69 PMID: 1201683
  11. Identification of the lampbrush chromosome loops which transcribe 5S ribosomal RNA in Notophthalmus (Triturus) viridescens.
    Chromosoma. 1975 Nov 20;53(1):71-89 PMID: 1201684
  12. Repeating units of Xenopus laevis oocyte-type 5S DNA are heterogeneous in length.
    Cell. 1976 Apr;7(4):467-75 PMID: 986247
  13. The molecular basis for length heterogeneity in ribosomal DNA from Xenopus laevis.
    J Mol Biol. 1976 Aug 25;105(4):461-86 PMID: 787539
  14. A simple method for DNA restriction site mapping.
    Nucleic Acids Res. 1976 Sep;3(9):2387-98 PMID: 787937
  15. Dictyostelium 17S, 25S, and 5S rDNAs lie within a 38,000 base pair repeated unit.
    Cell. 1976 Nov;9(3):431-8 PMID: 1033038
  16. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  17. Cloning and characterization of a complex satellite DNA from Drosophila melanogaster.
    Cell. 1977 Jun;11(2):371-81 PMID: 408008
  18. A pseudogene structure in 5S DNA of Xenopus laevis.
    Cell. 1977 Sep;12(1):109-20 PMID: 561661
  19. The use of thin acrylamide gels for DNA sequencing.
    FEBS Lett. 1978 Mar 1;87(1):107-10 PMID: 631324
  20. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  21. The nucleotide sequence of oocyte 5S DNA in Xenopus laevis. I. The AT-rich spacer.
    Cell. 1978 Apr;13(4):701-16 PMID: 566163
  22. The isolation of structural genes from libraries of eucaryotic DNA.
    Cell. 1978 Oct;15(2):687-701 PMID: 719759
  23. Nucleotide sequence of Xenopus borealis oocyte 5S DNA: comparison of sequences that flank several related eucaryotic genes.
    Cell. 1978 Dec;15(4):1145-56 PMID: 264240
  24. A 9.6 kb intervening sequence in D. virilis rDNA, and sequence homology in rDNA interruptions of diverse species of Drosophila and other diptera.
    Cell. 1979 Apr;16(4):763-75 PMID: 110456
  25. Filter replicas and permanent collections of recombinant DNA plasmids.
    Nucleic Acids Res. 1979 Dec 20;7(8):2115-36 PMID: 118435
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.23.6457
Published
1981-12-11
Pages
6457-69
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327616
Subset
IM
Grants
NIGMS NIH HHS · GM 12427 · United States
Databases
GENBANK
M13611
External Links
PubMed source DOI: 10.1093/nar/9.23.6457 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2