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PMID: 6274848 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Amino-terminal sequence of the Tn3 transposase protein.

Journal of bacteriology ·Vol. 149 ·No. 1 ·1982-01-00 ·Pages 407-10

Ditto MD, Chou J, Hunkapiller MW, Fennewald MA, Gerrard SP, Hood LE, Cohen SN, Casadaban MJ

Abstract

The amino-terminal sequence of the Tn3 transposase protein was determined to be Pro-Val-Asp-Phe-Leu-Thr-Thr-Glu-Gln-Val-Glu-Ser.... This was determined both from an active transposase protein purified from a transposase overproducing mutant strain and from a hybrid transposase-beta-galactosidase fusion protein. The amino acid sequence corresponded to the DNA sequence of the transposase gene beginning at an ATG initiation codon, as previously predicted from the analysis of transposase-beta-galactosidase gene fusions.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Codon DNA Transposable Elements DNA, Recombinant Nucleotidyltransferases/genetics Protein Biosynthesis Transposases
Chemicals
Bacterial Proteins Codon DNA Transposable Elements DNA, Recombinant Nucleotidyltransferases Transposases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Ditto M D
Chou J
Hunkapiller M W
Fennewald M A
Gerrard S P
Hood L E
Cohen S N
Casadaban M J
References (15)
15 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
Published
1982-01-00
Pages
407-10
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216644
Subset
IM
Grants
NIGMS NIH HHS · GM 06965 · United States
NIGMS NIH HHS · GM 29067 · United States
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