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PMID: 6272896 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The effect of tetramethylammonium on single sodium channel currents.

Biophysical journal ·Vol. 36 ·No. 2 ·1981-11-00 ·Pages 321-7

Horn R, Patlak J, Stevens CF

Abstract

Voltage-dependent Na conductance of rat myotubes was studied by patch recordings of single-channels. The patches were excised from the cell with the patch electrode, and the cytoplasmic surface was bathed in either CsF or tetramethylammonium (TMA)-F. Inward currents were examined from -20 to -50 mV. In this range Cs and TMA both appeared to be nearly impermeant, but TMA blocked the channel in a voltage-dependent manner. A first-order blocking site was located a maximum of 89% of the way through the membrane field from the cytoplasmic surface.

MeSH Terms
Animals Electric Conductivity Ion Channels/drug effects,metabolism Membrane Potentials/drug effects Muscles/metabolism,physiology Rats Sodium/metabolism Tetraethylammonium Compounds/pharmacology
Chemicals
Ion Channels Tetraethylammonium Compounds Sodium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Horn R
Patlak J
Stevens C F
References (23)
23 references, click to expand
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(81)84734-0
Published
1981-11-00
Pages
321-7
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1327598
Subset
IM
Grants
NIGMS NIH HHS · GM 24749 · United States
NINDS NIH HHS · NS 12961 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(81)84734-0 PMC Full Text View journal details
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