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PMID: 6257898 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Voltage-clamp experiments in normal and denervated mammalian skeletal muscle fibres.

The Journal of physiology ·Vol. 306 ·1980-09-00 ·Pages 377-410

Pappone PA

Abstract

1. The vaseline-gap voltage-clamp method has been applied to the study of ionic currents in cut pieces from innervated and 5--7 days denervated rat skeletal muscle fibres. 2. Kinetic analysis of sodium currents in innervated rat muscle showed them to be similar to those in frog muscle, except that rat sodium channels are activated at slightly more negative potentials. Peak sodium conductances of 40--50 m-mho/cm2 were measured, corresponding to values of GNa of 100--120 m-mho/cm2. 3. The permeability sequence of the sodium channel to several organic and inorganic cations is Li+ greater than Na+ greater than hydroxylammonium greater than hydrazinium greater than guanidinium approximately ammonium greater than K+. TMA+ and Ca2+ were not measurably permeant. 4. Denervation appears to shift activation and inactivation parameters of sodium currents by approximately 10 mV to more negative potentials, but does not appreciably affect the maximum peak sodium conductance or the time constants for activation and inactivation. 5. Dose--response curves for block by tetrodotoxin in innervated fibres are fitted well by assuming binding of toxin to a single population of channels with a dissociation constant of about 5 nM. In denervated fibres there appears in addition a second population of channels with a dissociation constant in the micromolar range. These relatively toxin-insensitive channels respond less rapidly to potential changes, and can contribute up to 25--30% of the total sodium conductance. 6. The potassium currents of innervated rat muscle were similar to those of frog muscle in their voltage dependence of activation. 7. The time constant for inactivation of the sodium current, tau h, at -13 mV showed a temperature dependence measured between 10 and 20 degrees C equivalent to an average Q10 of 2 . 3. The Q10 for the time constant of activation of the potassium current tau n, averaged 2 . 5 between -40 mV and +40 mV, measured over the same temperature range.

MeSH Terms
Animals Electric Conductivity In Vitro Techniques Ion Channels/physiology Kinetics Membrane Potentials Muscle Denervation Muscles/drug effects,innervation,physiology Potassium/physiology Rats Sodium/metabolism,physiology Tetrodotoxin/pharmacology
Chemicals
Ion Channels Tetrodotoxin Sodium Potassium
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Pappone P A
References (48)
48 references, click to expand
  1. Action potential generation in denervated rat skeletal muscle. II. The action of tetrodotoxin.
    Acta Physiol Scand. 1971 May;82(1):70-8 PMID: 4327036
  2. Membrane constants of red and white muscle fibers in the rat.
    Jpn J Physiol. 1967 Dec 15;17(6):720-5 PMID: 5300937
  3. Sodium movements in denervated muscle and the effects of antimycin A.
    J Physiol. 1968 Jul;197(2):279-94 PMID: 5716846
  4. Ionic currents in mammalian fast skeletal muscle.
    J Physiol. 1978 May;278:403-23 PMID: 671323
  5. Sodium currents in mammalian muscle.
    J Physiol. 1977 Jun;268(1):223-50 PMID: 874895
  6. Measurement of the conductance of the sodium channel from current fluctuations at the node of Ranvier.
    J Physiol. 1976 Nov;262(3):699-727 PMID: 1087643
  7. Reconstruction of the action potential of frog sartorius muscle.
    J Physiol. 1973 Nov;235(1):103-31 PMID: 4778131
  8. An improved vaseline gap voltage clamp for skeletal muscle fibers.
    J Gen Physiol. 1976 Mar;67(3):265-93 PMID: 1083424
  9. Action potentials reconstructed in normal and myotonic muscle fibres.
    J Physiol. 1976 Jun;258(1):125-43 PMID: 940049
  10. Impedance of frog skeletal muscle fibers in various solutions.
    J Gen Physiol. 1974 Apr;63(4):460-91 PMID: 4544879
  11. LINEAR ELECTRICAL PROPERTIES OF STRIATED MUSCLE FIBRES OBSERVED WITH INTRACELLULAR ELECTRODES.
    Proc R Soc Lond B Biol Sci. 1964 Apr 14;160:69-123 PMID: 14142170
  12. Effects of denervation and colchicine treatment on the chloride conductance of rat skeletal muscle fibers.
    J Neurobiol. 1976 May;7(3):221-8 PMID: 1271053
  13. Membrane capacity measurements on frog skeletal muscle in media of low ion content.
    J Physiol. 1974 Mar;237(3):573-605 PMID: 4545185
  14. Tetrodotoxin binding to normal depolarized frog muscle and the conductance of a single sodium channel.
    J Physiol. 1975 May;247(2):483-509 PMID: 1080198
  15. Chloride conductance in normal and myotonic muscle fibres and the action of monocarboxylic aromatic acids.
    J Physiol. 1971 Dec;219(2):367-83 PMID: 5316641
  16. Differential effects of tetracaine on delayed potassium channels and displacement currents in frog skeletal muscle.
    J Physiol. 1976 Nov;262(3):613-37 PMID: 1087642
  17. Kinetic properties of the sodium current in striated muscle fibres on the basis of the Hodgkin-Huxley theory.
    J Physiol. 1972 Dec;227(2):419-31 PMID: 4647253
  18. Voltage clamp experiments in striated muscle fibres.
    J Physiol. 1970 Jul;208(3):607-44 PMID: 5499787
  19. Cation movements in normal and short-term denervated rat fast twitch muscle.
    J Physiol. 1977 Oct;271(3):605-24 PMID: 926018
  20. The binding of tetrodotoxin and alpha-bungarotoxin to normal and denervated mammalian muscle.
    J Physiol. 1974 Jul;240(1):199-226 PMID: 4854666
  21. Anode break excitation in denervated rat skeletal muscle fibres.
    J Physiol. 1974 Jan;236(2):413-20 PMID: 16992443
  22. Kinetic and pharmacological properties of the sodium channel of frog skeletal muscle.
    J Gen Physiol. 1976 Mar;67(3):309-23 PMID: 4577
  23. A comparative study of membrane properties of innervated and chronically denervated fast and slow skeletal muscles of the rat.
    Acta Physiol Scand. 1968 Aug;73(4):471-80 PMID: 5708174
  24. Inhibition of denervation changes in skeletal muscle by blockers of protein synthesis.
    J Physiol. 1972 Mar;221(3):743-54 PMID: 5016370
  25. Characteristics of the chloride conductance in muscle fibers of the rat diaphragm.
    J Gen Physiol. 1977 Mar;69(3):325-42 PMID: 15046
  26. The binding of labelled saxitoxin to the sodium channels in normal and denervated mammalian muscle, and in amphibian muscle.
    J Physiol. 1977 Jul;269(2):341-54 PMID: 302332
  27. A quantitative description of membrane current and its application to conduction and excitation in nerve.
    J Physiol. 1952 Aug;117(4):500-44 PMID: 12991237
  28. On the repetitive discharge in myotonic muscle fibres.
    J Physiol. 1974 Jul;240(2):505-15 PMID: 4420758
  29. The effect of diameter on the electrical constants of frog skeletal muscle fibres.
    J Physiol. 1972 Feb;221(1):105-20 PMID: 4536963
  30. Voltage and time dependence of excitatory sodium current in cooled sheep Purkinje fibres.
    Pflugers Arch. 1970;315(2):136-58 PMID: 5460932
  31. Ionic selectivity of the sodium channel of frog skeletal muscle.
    J Gen Physiol. 1976 Mar;67(3):295-307 PMID: 1262852
  32. Voltage-clamp experiments on frog single skeletal muscle fibres: evidence for a tubular sodium current.
    J Physiol. 1977 Aug;269(3):605-25 PMID: 302334
  33. The inner quaternary ammonium ion receptor in potassium channels of the node of Ranvier.
    J Gen Physiol. 1972 Apr;59(4):388-400 PMID: 4112955
  34. Action potential in the transverse tubules and its role in the activation of skeletal muscle.
    J Gen Physiol. 1974 Feb;63(2):257-78 PMID: 4812638
  35. Changes in the sarcoplasmic reticulum and transverse tubular system of fast and slow skeletal muscles of the mouse during postnatal development.
    J Cell Biol. 1971 Nov;51(21):369-83 PMID: 5112650
  36. Pharmacological modifications of the sodium channels of frog nerve.
    J Gen Physiol. 1968 Feb;51(2):199-219 PMID: 5641635
  37. The kinetics of mechanical activation in frog muscle.
    J Physiol. 1969 Sep;204(1):207-30 PMID: 5352046
  38. Membrane constants of muscle fibers of rat diaphragm.
    Am J Physiol. 1970 Sep;219(3):654-7 PMID: 4247498
  39. Membrane properties underlying spontaneous activity of denervated muscle fibres.
    J Physiol. 1974 May;239(1):125-53 PMID: 4853156
  40. Stereological analysis of mammalian skeletal muscle. II. White vastus muscle of the adult guinea pig.
    J Ultrastruct Res. 1975 May;51(2):176-87 PMID: 1127796
  41. Contractile activation by voltage clamp depolarization of cut skeletal muscle fibres.
    J Physiol. 1978 Apr;277:483-506 PMID: 306440
  42. Fast and slow mammalian muscles after denervation.
    Exp Neurol. 1970 Jan;26(1):183-202 PMID: 4312351
  43. Membrane currents in isolated frog nerve fibre under voltage clamp conditions.
    J Physiol. 1958 Aug 29;143(1):76-90 PMID: 13576461
  44. Studies on tetrodotoxin resistant action potentials in denervated skeletal muscle.
    Acta Physiol Scand. 1971 Nov;83(3):382-8 PMID: 5134176
  45. Local anesthetics. Effect of pH on use-dependent block of sodium channels in frog muscle.
    Biophys J. 1977 Dec;20(3):343-68 PMID: 21711
  46. Action potential generation in denervated rat skeletal muscle. I. Quantitative aspects.
    Acta Physiol Scand. 1971 Apr;81(4):557-64 PMID: 5091113
  47. Saxitoxin binding to sodium channels of rat skeletal muscles.
    J Physiol. 1980 Mar;300:89-103 PMID: 6247488
  48. The effect of sodium ions on the electrical activity of giant axon of the squid.
    J Physiol. 1949 Mar 1;108(1):37-77 PMID: 18128147
Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.1980.sp013403
Published
1980-09-00
Pages
377-410
Language
English
Region
England
NLM ID
0266262
PMCID
PMC1283012
Subset
IM
Grants
NIADDK NIH HHS · AM17803 · United States
NIGMS NIH HHS · GMO7270 · United States
External Links
PubMed source DOI: 10.1113/jphysiol.1980.sp013403 PMC Full Text View journal details
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