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PMID: 6271732 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Cloning of immunity and structural genes for colicin V.

Journal of bacteriology ·Vol. 148 ·No. 2 ·1981-11-00 ·Pages 498-507

Frick KK, Quackenbush RL, Konisky J

Abstract

The colicin V immunity and structural genes of plasmid pColV-B188 were cloned into the vectors pMB9, pBR322, and pMK16. Both genes are closely linked and can be isolated on a 900-base-pair deoxyribonucleic acid fragment. Insertion of the transposon Tn5 into this cloned sequence led to the construction of a mutant plasmid which conferred colicin V immunity, but not the ability to produce this colicin. Analysis of the products determined by these cloned genes in cells has led to the conclusion that the polypeptide involved in immunity has a molecular weight of about 6,500, whereas the colicin has a molecular weight of approximately 4,000.

MeSH Terms
Bacteriocin Plasmids Cloning, Molecular Colicins/biosynthesis,pharmacology DNA Transposable Elements Escherichia coli/genetics Genes Genes, Bacterial Molecular Weight Plasmids
Chemicals
Colicins DNA Transposable Elements
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Frick K K
Quackenbush R L
Konisky J
References (28)
28 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.148.2.498-507.1981
Published
1981-11-00
Pages
498-507
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216232
Subset
IM
Grants
NIAID NIH HHS · 1P32 AI05645-01 · United States
NIAID NIH HHS · AI-1088 · United States
NIAID NIH HHS · AI10106 · United States
External Links
PubMed source DOI: 10.1128/jb.148.2.498-507.1981 PMC Full Text View journal details
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