Home LiteratureArticle Details
PMID: 6157671 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Kinetics and regulation of cell-free alkaline phosphatase synthesis.

Journal of bacteriology ·Vol. 143 ·No. 3 ·1980-09-00 ·Pages 1265-74

Pratt C

Abstract

Regulation of alkaline phosphatase (EC 3.1.3.1) synthesis in a cell-free system from Escherichia coli has been observed. Synthesis from transducing phage deoxyribonucleic acid templates carrying phoA+ occurred in S30 fractions from wild-type or alkaline phosphatase-constitutive mutants. It did not occur in S30) fractions from alkaline phosphatase-negative mutants (phoB). The hybrid gene phoA-lacZ was also subject to phoB control, implying that phoA transcription is regulated. The yield of active alkaline phosphatase per phoA+ gene copy from cell-free synthesis was similar to that of beta-galactosidase. Alkaline phosphatase activity took longer to appear than beta-galactosidase activity. Synthesis of alkaline phosphatase subunits was not delayed, suggesting that a minimum number of subunits are synthesized before formation of active alkaline phosphatase occurs.

MeSH Terms
Alkaline Phosphatase/biosynthesis Cell-Free System Escherichia coli/enzymology,genetics Genes, Regulator Kinetics Mutation Phosphates/metabolism RNA, Bacterial/biosynthesis RNA, Messenger/biosynthesis
Chemicals
Phosphates RNA, Bacterial RNA, Messenger Alkaline Phosphatase
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Pratt C
References (42)
42 references, click to expand
  1. The reversible dissociation of the alkaline phosphatase of Escherichia coli. I. Formation and reactivation of subunits.
    J Biol Chem. 1965 Nov;240(11):4284-92 PMID: 4954367
  2. Mode of action of rafamycin on the RNA polymerase reaction.
    Biochim Biophys Acta. 1968 Mar 18;157(1):218-9 PMID: 4868249
  3. Purification and properties of intracellular lamba DNA rings.
    J Mol Biol. 1968 Apr 28;33(2):395-413 PMID: 4885485
  4. The non-linear relationship between the enzyme activity and structural protein concentration of thiogalactoside transacetylase of E. coli.
    Biochem Biophys Res Commun. 1969 Feb 21;34(4):522-7 PMID: 4887461
  5. The step sensitive to catabolite repression and its reversal by 3'-5' cyclic AMP during induced synthesis of beta-galactosidase in E. coli.
    Biochem Biophys Res Commun. 1969 Jul 7;36(1):84-92 PMID: 4307746
  6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  7. DNA-dependent in vitro synthesis of enzymes of the galactose operon of Escherichia coli.
    Mol Gen Genet. 1971;112(1):14-27 PMID: 4330068
  8. Physiological factors in the regulation of alkaline phosphatase synthesis in Escherichia coli.
    J Bacteriol. 1972 May;110(2):616-23 PMID: 4553839
  9. Detection and isolation of the repressor protein for the tryptophan operon of Escherichia coli.
    Proc Natl Acad Sci U S A. 1972 May;69(5):1100-3 PMID: 4338582
  10. Inorganic phosphate transport in Escherichia coli: involvement of two genes which play a role in alkaline phosphatase regulation.
    J Bacteriol. 1973 Feb;113(2):529-39 PMID: 4570598
  11. A ne type of alkaline phosphatase-negative mutants in Escherichia coli K12.
    Mol Gen Genet. 1973 Mar 27;122(1):53-60 PMID: 4573262
  12. In vitro synthesis of protein in microbial systems.
    Annu Rev Genet. 1973;7:267-87 PMID: 4593305
  13. Regulation of the L-arabinose operon BAD in vitro.
    J Biol Chem. 1974 May 10;249(9):2946-52 PMID: 4364034
  14. The relationship between the phosphate-binding protein and a regulator gene product from Escherichia coli.
    Biochim Biophys Acta. 1974 May 10;351(1):77-86 PMID: 4599976
  15. Culture medium for enterobacteria.
    J Bacteriol. 1974 Sep;119(3):736-47 PMID: 4604283
  16. Pleiotropic effects of mutations involved in the regulation of Escherichia coli K-12 alkaline phosphatase.
    J Bacteriol. 1974 Aug;119(2):583-92 PMID: 4212247
  17. Coordinate and differential in vitro syntheses of two RNA polymerase subunits.
    Nature. 1974 Dec 13;252(5484):596-7 PMID: 4610425
  18. Analysis of genetic regulatory mechanisms.
    Annu Rev Genet. 1974;8:1-13 PMID: 4613253
  19. Analysis of the regulation of Escherichia coli alkaline phosphatase synthesis using deletions and phi80 transducing phages.
    J Mol Biol. 1975 Aug 5;96(2):307-16 PMID: 1100846
  20. Positive control of D-serine deaminase synthesis in vitro.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):1695-9 PMID: 347444
  21. Use of gene fusions to determine a partial signal sequence of alkaline phosphatase.
    J Bacteriol. 1979 Sep;139(3):932-9 PMID: 113391
  22. Escherichia coli pleiotropic mutant that reduces amounts of several periplasmic and outer membrane proteins.
    J Bacteriol. 1979 Oct;140(1):229-39 PMID: 387722
  23. The localization of alkaline phosphatase in E. coli K12.
    Biochem Biophys Res Commun. 1961 Jun 2;5:104-8 PMID: 13765699
  24. Genetic control of induction of alkaline phosphatase synthesis in E. coli.
    Proc Natl Acad Sci U S A. 1962 Aug;48:1398-402 PMID: 13896931
  25. Properties of two regulating genes for alkaline phosphatase.
    J Bacteriol. 1962 Feb;83:297-300 PMID: 13896932
  26. Genetic evidence on the nature of the repressor for alkaline phosphatase in E. coli.
    J Mol Biol. 1963 May;6:433-8 PMID: 13946565
  27. ISOLATION OF A PROTEIN SPECIFIED BY A REGULATOR GENE.
    J Mol Biol. 1964 Jun;8:841-52 PMID: 14192076
  28. THE PUROMYCIN REACTION AND ITS RELATION TO PROTEIN SYNTHESIS.
    J Mol Biol. 1964 Oct;10:63-72 PMID: 14222897
  29. Influence of inorganic phosphate in the formation of phosphatases by Escherichia coli.
    Biochim Biophys Acta. 1960 Mar 11;38:460-9 PMID: 13838951
  30. Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.
    J Cell Biol. 1975 Dec;67(3):835-51 PMID: 811671
  31. Genetic analysis of regulatory mutants of alkaline phosphatase of E. coli.
    Genetics. 1975 Nov;81(3):459-68 PMID: 1107145
  32. In vitro synthesis and repression of argininosuccinase in Escherichia coli K12; partial purification of the arginine repressor.
    Mol Gen Genet. 1976 Feb 27;144(1):17-20 PMID: 772411
  33. Recalibrated linkage map of Escherichia coli K-12.
    Bacteriol Rev. 1976 Mar;40(1):116-67 PMID: 773363
  34. Control of the synthesis of alkaline phosphatase and the phosphate-binding protein in Escherichia coli.
    J Bacteriol. 1976 Jul;127(1):595-609 PMID: 179980
  35. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu.
    J Mol Biol. 1976 Jul 5;104(3):541-55 PMID: 781293
  36. Positions of single-strand breaks in lambda DNA.
    Biochim Biophys Acta. 1977 Jan 3;474(1):44-8 PMID: 831810
  37. Alkaline phosphatase synthesis in a cell-free system using DNA and RNA templates.
    J Mol Biol. 1977 Feb 15;110(1):75-87 PMID: 845948
  38. Synthesis and processing of an Escherichia coli alkaline phosphatase precursor in vitro.
    Proc Natl Acad Sci U S A. 1977 Apr;74(4):1440-4 PMID: 323853
  39. Complementation test between alkaline phosphatase regulatory mutations phoB and phoRc in Escherichia coli.
    Genetics. 1977 Feb;85(2):203-8 PMID: 324866
  40. Transcription termination at the end of the tryptophan operon of Escherichia coli.
    J Mol Biol. 1977 May 25;112(3):423-36 PMID: 327077
  41. Extracellular labeling of nascent polypeptides traversing the membrane of Escherichia coli.
    Proc Natl Acad Sci U S A. 1977 Jul;74(7):2830-4 PMID: 331317
  42. Regulation of argA operon expression in Escherichia coli K-12: cell-free synthesis of beta-galactosidase under argA control.
    J Bacteriol. 1977 Oct;132(1):67-72 PMID: 410786
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.143.3.1265-1274.1980
Published
1980-09-00
Pages
1265-74
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC294493
Subset
IM
Grants
NIGMS NIH HHS · R01-GM-24009-03 · United States
External Links
PubMed source DOI: 10.1128/jb.143.3.1265-1274.1980 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2