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PMID: 6270662 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Molecular cloning and partial characterization of unintegrated linear DNA from gibbon ape leukemia virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 7 ·1981-07-00 ·Pages 4213-7

Scott ML, McKereghan K, Kaplan HS, Fry KE

Abstract

We have cloned the complete genome of an oncogenic primate retrovirus, the San Francisco isolate of gibbon ape leukemia virus, in a lambda phage vector. DNA sequence analysis and restriction endonuclease mapping of the inserted linear provirus demonstrated 9-base pair inverted repeats at its ends, flanking direct terminal repeats 470 base pairs in length. The (-) strong stop region of this DNA showed surprisingly low sequence homology to that of another gibbon ape leukemia virus isolate from an animal with similar disease. Analysis of the clone also revealed the terminal phosphate configuration of the linear provirus. The recombinant phage is suitable for direct use as a hybridization probe to detect homologous retroviral sequences in human cell lines.

MeSH Terms
Animals Bacteriophage lambda Base Sequence Cell Transformation, Viral Cloning, Molecular/methods DNA, Viral/genetics Hominidae/microbiology Hylobates/microbiology Repetitive Sequences, Nucleic Acid Retroviridae/genetics
Chemicals
DNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Scott M L
McKereghan K
Kaplan H S
Fry K E
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24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.7.4213
Published
1981-07-00
Pages
4213-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319759
Subset
IM
Grants
NCI NIH HHS · CA-29079 · United States
NCI NIH HHS · N0I-CP-91044 · United States
NCRR NIH HHS · P41RR-00785-07 · United States
Databases
GENBANK
J02196
External Links
PubMed source DOI: 10.1073/pnas.78.7.4213 PMC Full Text View journal details
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