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PMID: 6268938 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The structure of R1drd19: a revised physical map of the plasmid.

Molecular & general genetics : MGG ·Vol. 181 ·No. 2 ·1981-00-00 ·Pages 183-91

Clerget M, Chandler M, Caro L

Abstract

We have analyzed derivatives of the plasmid R1drd19 carrying the transposon Tn10 by electron microscopy following denaturation and renaturation of the molecules, and by digestion with various restriction enzymes, gel electrophoresis and Southern blotting. We show: 1) that the published restriction map of R1drd19 is inconsistent with our results. We present a modified map which is consistent with our data. 2) that R1drd19 carries a single resident copy of the element IS10 which is normally associated with Tn10 as an inverted repeat, and 3) that R1drd19 carries three copies of the insertion element IS1 in the resistance determinant region.

MeSH Terms

DNA Restriction Enzymes DNA Transposable Elements Escherichia coli/genetics Microscopy, Electron Nucleic Acid Hybridization R Factors Repetitive Sequences, Nucleic Acid

Chemicals

DNA Transposable Elements DNA Restriction Enzymes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Clerget M

Chandler M

Caro L

References (27)

27 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00268425

Published

1981-00-00

Pages

183-91

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00268425

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The structure of R1drd19: a revised physical map of the plasmid.

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