Tn10 mediated integration of the plasmid R100.1 into the bacterial chromosome: inverse transposition.

Molecular & general genetics : MGG ·Vol. 173 ·No. 1 ·1979-05-23 ·Pages 23-30

Chandler M, Roulet E, Silver L, Boy de la Tour E, Caro L

Abstract

Upon integration into the bacterial chromosome the drug resistance plasmid R100.1 often loses its tetracycline resistance character. We have analyzed an Hfr strain formed by such an integration and an R-prime plasmid derived from it. We find that integration took place within the Tn10 transposon, that the two IS10 sequences were retained, but that at least 80% of the transposon segment located between them, and carrying the tetracycline resistance genes, had been lost. We suggest that integration of R100.1 was mediated by an inverse transposition using the IS10 sequences.

MeSH Terms

Chromosomes, Bacterial Escherichia coli/genetics Phenotype R Factors Recombination, Genetic Tetracycline/pharmacology

Chemicals

Tetracycline

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Chandler M

Roulet E

Silver L

Boy de la Tour E

Caro L

References (27)

27 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00267687

Published

1979-05-23

Pages

23-30

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00267687

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Tn10 mediated integration of the plasmid R100.1 into the bacterial chromosome: inverse transposition.

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