Isolation of Hfr strains from R+ and ColV2+ strains of Escherichia coli and derivation of an R'lac factor by transduction.

Journal of bacteriology ·Vol. 116 ·No. 3 ·1973-12-00 ·Pages 1107-12

Nishimura A, Nishimura Y, Caro L

Abstract

Temperature-resistant revertants were isolated from Escherichia coli strains carrying a temperature-sensitive dnaA mutation (initiation of chromosome replication) and either a repressed or a derepressed F-like R factor or a ColV2 factor. Many of the revertants had all the properties of Hfr strains, with a variety of directions and origins of transfer. From one such revertant, episomes carrying the R factor and part of the lac region (R'lac) could be isolated by transduction. This system offers a good selection for Hfr strains produced by integration of various episomes and for the isolation of R' factors.

MeSH Terms

Anti-Bacterial Agents/pharmacology Chromosome Mapping Colicins/biosynthesis DNA Replication DNA, Bacterial/biosynthesis Drug Resistance, Microbial Escherichia coli/drug effects,isolation & purification,metabolism Genes, Regulator Mutation Suppression, Genetic Temperature Transduction, Genetic

Chemicals

Anti-Bacterial Agents Colicins DNA, Bacterial

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Nishimura A

Nishimura Y

Caro L

References (10)

10 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.116.3.1107-1112.1973

Published

1973-12-00

Pages

1107-12

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC246463

Subset

External Links

PubMed source DOI: 10.1128/jb.116.3.1107-1112.1973 PMC Full Text View journal details

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Isolation of Hfr strains from R+ and ColV2+ strains of Escherichia coli and derivation of an R'lac factor by transduction.

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