Home LiteratureArticle Details
PMID: 6268923 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Effect of diabetes and insulin replacement on the lipid properties of hepatic smooth endoplasmic reticulum.

Lipids ·Vol. 16 ·No. 7 ·1981-07-00 ·Pages 525-32

Holloway CT, Garfield SA

Abstract

This study is a characterization of the lipid properties of the smooth and rough endoplasmic reticulum (SER, RER) of liver from streptozotocin-induced diabetic rats. A significant decrease in membrane microviscosity was observed in the SER but not the RER of diabetic rats when compared to that of normal controls. This decrease in SER membrane microviscosity correlated with a decrease in cholesterol/phospholipid ratio of these membranes that could be accounted for solely by a change in the membrane cholesterol content. Changes in phospholipid fatty acyl chain composition were also observed in the SER membranes but these changes were small when compared to the large change in cholesterol content observed. Insulin treatment for only one day did not significantly alter the microviscosity of the SER but after 2, 4 and 6 days of treatment both membrane microviscosity and membrane cholesterol content were restored to values similar to those for normal animals. No significant changes in the RER lipid composition were observed. It is well known that increases in glucose-6-Pase (G-6-Pase) activity of liver ER membranes are associated with diabetic onset. An increase in the specific activity of G-6-Pase was observed in both SER and RER membrane preparations, although the observed increase in the SER membrane is higher. The changes in the G-6-Pase activity of the SER membranes were correlated with the alterations in the microviscosity and lipid composition of these membranes. It is postulated that lipid properties of the SER membranes may contribute to the regulation of G-6-Pase activity in that membrane.

MeSH Terms
Animals Cholesterol/analysis Diabetes Mellitus, Experimental/drug therapy,metabolism Endoplasmic Reticulum/metabolism Fatty Acids/analysis Glucose-6-Phosphatase/metabolism Insulin/therapeutic use Lipid Metabolism Lipids/analysis Liver/metabolism Male Phospholipids/analysis Rats Viscosity
Chemicals
Fatty Acids Insulin Lipids Phospholipids Cholesterol Glucose-6-Phosphatase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Holloway C T
Garfield S A
References (39)
39 references, click to expand
  1. Modulation of hexose uptake and insulin action by cell membrane fluidity. The effects of temperature on membrane fluidity, insulin action, and insulin binding.
    J Biol Chem. 1979 Apr 25;254(8):2619-25 PMID: 218948
  2. Hepatic malonyl-CoA levels of fed, fasted and diabetic rats as measured using a simple radioisotopic assay.
    J Biol Chem. 1978 Nov 25;253(22):8291-3 PMID: 711752
  3. Adaptive alteration in phospholipid composition of plasma membranes from a somatic cell mutant defective in the regulation of cholesterol biosynthesis.
    J Cell Biol. 1980 Apr;85(1):166-9 PMID: 7364872
  4. Effect of insulin on the oxidative desaturation ofa-linolenic, oleic and palmitic acids.
    Lipids. 1967 May;2(3):284-5 PMID: 17805784
  5. Factors influencing the lipid composition and fluidity of red cell membranes in vitro: production of red cells possessing more than two cholesterols per phospholipid.
    Biochemistry. 1978 Jan 24;17(2):327-31 PMID: 619993
  6. Microviscosity parameters and protein mobility in biological membranes.
    Biochim Biophys Acta. 1976 Apr 16;433(1):133-49 PMID: 1260056
  7. The molecular organization of membranes.
    Annu Rev Biochem. 1974;43(0):805-33 PMID: 4277710
  8. Biosynthetic pathways of polyunsaturated fatty acids.
    Adv Exp Med Biol. 1977;83:35-50 PMID: 920469
  9. Gross structural changes in isolated liver cell plasma membranes upon binding of insulin.
    Biochemistry. 1979 Feb 6;18(3):445-50 PMID: 420790
  10. The influence of hormonal factors and of diet upon hepatic glucose-6-phosphatase activity.
    J Biol Chem. 1955 May;214(1):167-74 PMID: 14367375
  11. Fatty acid desaturation in experimental diabetes mellitus.
    Diabetes. 1979 May;28(5):479-85 PMID: 437377
  12. Fluidity parameters of lipid regions determined by fluorescence polarization.
    Biochim Biophys Acta. 1978 Dec 15;515(4):367-94 PMID: 365237
  13. The influence of cholesterol on the activity, on the isothermic kinetics and on the temperature-induced kinetics of 3-hydroxy-3-methylglutaryl coenzyme a reductase.
    Biochim Biophys Acta. 1977 Oct 24;489(1):126-42 PMID: 911871
  14. The effect of experimental diabetes mellitus and insulin replacement on hepatic ultrastructure and protein synthesis.
    J Clin Invest. 1973 Feb;52(2):248-62 PMID: 4683872
  15. Different properties of glucose-6-phosphatase and related enzymes in rough and smooth endoplasmic reticular membranes.
    Biochim Biophys Acta. 1971 Mar 9;233(1):163-75 PMID: 4325419
  16. Sensitivity of the adipocyte D-glucose transport system to membrane fluidity in reconstituted vesicles.
    J Biol Chem. 1979 Sep 25;254(18):8744-7 PMID: 479153
  17. Fluidity of LM cell membranes with modified lipid compositions as determined with 1,6-diphenyl-1,3,5-hexatriene.
    Biochemistry. 1979 Mar 20;18(6):1042-9 PMID: 218610
  18. Quantitative aspects of relationship between glucose 6-phosphate transport and hydrolysis for liver microsomal glucose-6-phosphatase system. Selective thermal inactivation of catalytic component in situ at acid pH.
    J Biol Chem. 1976 Nov 10;251(21):6784-90 PMID: 10305
  19. THE EFFECT OF DIABETES AND FASTING ON LIVER GLUCOSE-6-PHOSPHATASE.
    Proc Natl Acad Sci U S A. 1954 Aug;40(8):673-8 PMID: 16589537
  20. The effect of insulin on monounsaturated fatty acid synthesis in diabetic rats. The stability of the informational RNA and of the enzyme system concerned with fatty acid desaturation.
    Biochim Biophys Acta. 1966 Jun 1;116(3):460-6 PMID: 5963011
  21. Regulation of rat liver beta-hydroxy-beta-methylglutaryl coenzyme. A reductase activity and cholesterol levels of serum and liver in various dietary and hormonal states.
    Arch Biochem Biophys. 1975 May;168(1):89-95 PMID: 1137403
  22. Dynamic lipid changes in rapidly proliferating hepatic smooth endoplasmic reticulum during acute dexamethasone treatment of adrenalectomized rats.
    Lipids. 1980 Dec;15(12):1037-43 PMID: 7219073
  23. A spin-label study of the role of phospholipids in the regulation of membrane-bound microsomal enzymes.
    J Mol Biol. 1973 Aug 5;78(2):351-62 PMID: 4355832
  24. A rapid method of total lipid extraction and purification.
    Can J Biochem Physiol. 1959 Aug;37(8):911-7 PMID: 13671378
  25. Effect of sterol depletion on LM cell sterol mutants. Changes in the lipid composition of the plasma membrane and their effects on 3-O-methlglucose transport.
    J Biol Chem. 1979 Feb 25;254(4):1108-13 PMID: 762119
  26. Use of a fluorescent probe to compare the plasma membrane properties in normal and transformed cells. Evaluation of the interference by triacylglycerols and alkyldiacylglycerols.
    Biochemistry. 1978 May 16;17(10):1997-2004 PMID: 207306
  27. Hepatic glucose-6-phosphatase activities and correlated ultrastructural alterations in hepatocytes of diabetic rats.
    Diabetes. 1979 Jul;28(7):664-79 PMID: 221299
  28. A simple method for the isolation and purification of total lipides from animal tissues.
    J Biol Chem. 1957 May;226(1):497-509 PMID: 13428781
  29. Use of protein-mediated lipid exchange in the study of membrane-bound enzymes. The lipid dependence of glucose-6-phosphatase.
    Eur J Biochem. 1979 Sep;99(3):605-12 PMID: 227688
  30. Rat liver microsomal elongation of fatty acids. Possible involvement of cytochrome b5.
    J Biol Chem. 1979 Aug 25;254(16):7778-84 PMID: 468787
  31. On the involvement of a glucose 6-phosphate transport system in the function of microsomal glucose 6-phosphatase.
    Mol Cell Biochem. 1975 Feb 28;6(2):75-83 PMID: 235736
  32. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  33. The effects of estradiol on stearyl-CoA desaturase activity and microsomal membrane properties in rooster liver.
    J Biol Chem. 1979 Mar 25;254(6):2004-9 PMID: 33993
  34. Dynamics of the hydrocarbon layer in liposomes of lecithin and sphingomyelin containing dicetylphosphate.
    J Biol Chem. 1974 Apr 25;249(8):2652-7 PMID: 4822508
  35. Lipid organization of the membrane of vesicular stomatitis virus.
    J Biol Chem. 1978 Jul 10;253(13):4544-50 PMID: 207703
  36. Regulatory function of delta6 desaturate -- key enzyme of polyunsaturated fatty acid synthesis.
    Adv Exp Med Biol. 1977;83:85-101 PMID: 200115
  37. Stimulation by insulin of rat liver -hydroxy- -methylglutaryl coenzyme A reductase and cholesterol-synthesizing activities.
    Biochem Biophys Res Commun. 1973 Feb 5;50(3):704-10 PMID: 4689073
  38. Colorimetric assay methods for free and phosphorylated glyceric acids.
    J Biol Chem. 1959 Mar;234(3):469-71 PMID: 13641242
  39. STUDIES ON THE STRUCTURAL AND ENZYMIC ORGANIZATION OF THE MEMBRANOUS ELEMENTS OF LIVER MICROSOMES.
    Acta Pathol Microbiol Scand Suppl. 1963;:SUPPL166:1-94 PMID: 14080472
Article Info
Journal
Lipids
Abbr.
Lipids
ISSN
0024-4201
DOI
10.1007/BF02535051
Published
1981-07-00
Pages
525-32
Language
English
Region
United States
NLM ID
0060450
Subset
IM
Grants
NIADDK NIH HHS · 5P60AM22125 · United States
NIADDK NIH HHS · AM 26873 · United States
NHLBI NIH HHS · HL 19936 · United States
External Links
PubMed source DOI: 10.1007/BF02535051 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2