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PMID: 6267260 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The influence of concanavalin A on glutamate-induced current fluctuations in locust muscle fibres.

The Journal of physiology ·Vol. 312 ·1981-03-00 ·Pages 1-8

Mathers DA

Abstract

1. The influence of the plant lectin Concanavalin A (ConA) on the properties of membrane channels opened by 25-200 microM-L-glutamate in voltage-clamped locust muscle fibres has been studied. Power spectral density plots of membrane current fluctuations were used to estimate the mean lifetime and conductance of glutamate-induced channels. 2. Exposure to ConA (10 micrograms/ml.) increased the amplitude of the mean current evoked by 200 microM-glutamate from 7.7 +/- 4.7 nA (mean +/- S.D.) to 81 +/- 57 nA (membrane potential -60 mV, temperature 16 degrees C). The mean conductance of glutamate-induced channels (65 +/- 16.8 pS at 16 degrees C) was negligibly affected by the lectin. 3. In normal saline the mean lifetime of glutamate-induced channels tau noise decreased on membrane hyperpolarization. After treatment with ConA (70 micrograms/ml.), tau noise become totally independent of membrane voltage when studied at potentials in the range -60 to -120 mV. The value of tau noise at the resting potential was little altered by the lectin.

MeSH Terms
Animals Concanavalin A/pharmacology Electric Conductivity Glutamates/physiology Grasshoppers In Vitro Techniques Ion Channels/drug effects Membrane Potentials/drug effects Muscles/drug effects,physiology
Chemicals
Glutamates Ion Channels Concanavalin A
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Mathers D A
References (37)
37 references, click to expand
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Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.1981.sp013611
Published
1981-03-00
Pages
1-8
Language
English
Region
England
NLM ID
0266262
PMCID
PMC1275536
Subset
IM
External Links
PubMed source DOI: 10.1113/jphysiol.1981.sp013611 PMC Full Text View journal details
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