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PMID: 6267161 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Calcium-regulatory mechanisms. Functional classification using skinned fibers.

The Journal of general physiology ·Vol. 77 ·No. 2 ·1981-02-00 ·Pages 177-90

Kerrick WG, Hoar PE, Cassidy PS, Bolles L, Malencik DA

Abstract

The primary purpose of this study was to determine whether various agents (adenosine 3-thiotriphosphate [ATP gamma S], trifluoperazine [TFP], troponin I, the catalytic subunit of the cyclic adenosine 3',5'-monophosphate dependent protein kinase [C-subunit], and calmodulin [CaM]) could be used to classify skinned fiber types, and then to determine whether the proposed mechanisms for Ca2+ regulation were consistent with the results. Agents (ATP gamma S, TFP, C-subunit, CaM) expected to alter a light chain kinase-phosphatase system strongly affect the Ca2+-activated tension in skinned gizzard smooth muscle fibers, whereas these agents have no effect on skinned mammalian striated and scallop adductor fibers. Troponin I, which is known to bind strongly to troponin C and CaM, inhibits Ca2+ activation of skinned mammalian striated and gizzard fibers but not scallop adductor muscle. The results in different types of skinned fibers are consistent with proposed mechanisms for Ca2+ regulation.

MeSH Terms
Adenosine Triphosphate/pharmacology Animals Calcium/physiology Calmodulin/pharmacology Cats Chickens Cyclic AMP/pharmacology In Vitro Techniques Muscle Contraction/drug effects Muscles/physiology Phenothiazines/pharmacology Protein Kinases/pharmacology Rabbits Troponin/pharmacology
Chemicals
Calmodulin Phenothiazines Troponin Adenosine Triphosphate Cyclic AMP Protein Kinases Calcium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kerrick W G
Hoar P E
Cassidy P S
Bolles L
Malencik D A
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38 references, click to expand
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Article Info
Journal
The Journal of general physiology
Abbr.
J Gen Physiol
ISSN
0022-1295
DOI
10.1085/jgp.77.2.177
Published
1981-02-00
Pages
177-90
Language
English
Region
United States
NLM ID
2985110R
PMCID
PMC2215427
Subset
IM
Grants
NIADDK NIH HHS · AM 07902 · United States
External Links
PubMed source DOI: 10.1085/jgp.77.2.177 PMC Full Text View journal details
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