Home LiteratureArticle Details
PMID: 6264456 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Fructose-bisphosphatase as a substrate of cyclic AMP-dependent protein kinase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 1 ·1981-01-00 ·Pages 91-4

Hosey MM, Marcus F

Abstract

We have tested rat liver fructose-bisphosphatase (D-fructose-1,6-bisphosphate 1-phosphohydrolase, EC 3.1.3.11) and three other gluconeogenic fructose-bisphosphatases as substrates for the catalytic subunit of cyclic AMP-dependent protein kinase. In contrast to the rat liver enzyme, homogeneous preparations of mouse liver, rabbit liver, and pig kidney fructose-bisphosphatase could not be phosphorylated by the kinase. Comparative sodium dodecyl sulfate/polyacrylamide gel electrophoresis of the four above fructose-bisphosphatases revealed that the subunit molecular weight of the isolated rat liver enzyme (ca. 40,000-42,000) was greater than that of mouse liver, rabbit liver, and pig kidney fructose-bisphosphatases (ca. 36,000-37,000). Treatment of 32P-labeled rat liver fructose-bisphosphatase with trypsin resulted in the conversion of the rat liver enzyme to an active species with a subunit molecular weight identical to that of the three other enzymes, with complete loss of the 32P-labeled site. Identical trypsin treatment of pig kidney fructose-bisphosphatase caused no change in the molecular weight of the enzyme. The results suggest that the purified mouse liver, rabbit liver, and pig kidney fructose-bisphosphatases are not substrates for the cyclic AMP-dependent protein kinase in vitro because they lack the phosphorylation-site peptide.

MeSH Terms
Animals Binding Sites Cyclic AMP/metabolism Fructose-Bisphosphatase/metabolism Kidney/enzymology Liver/enzymology Mice Peptide Fragments/metabolism Phosphorylation Protein Kinases/metabolism Rabbits Rats Substrate Specificity Swine Trypsin
Chemicals
Peptide Fragments Cyclic AMP Protein Kinases Fructose-Bisphosphatase Trypsin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hosey M M
Marcus F
References (24)
24 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Phosphorylation of rat hepatic fructose-1,6-bisphosphatase and pyruvate kinase.
    J Biol Chem. 1980 Apr 10;255(7):2770-5 PMID: 6244292
  3. Fructose-1,6-diphosphatase from rat liver. Purification and properties.
    Biochim Biophys Acta. 1974 Mar 21;341(1):129-37 PMID: 4364115
  4. The fructose 1,6-diphosphatase-phosphofructokinase substrate cycle. A site of regulation of hepatic gluconeogenesis by glucagon.
    J Biol Chem. 1974 Sep 25;249(18):5695-703 PMID: 4370466
  5. Fructose 1,6-bisphosphatase: properties of the neutral enzyme and its modification by proteolytic enzymes.
    Adv Enzymol Relat Areas Mol Biol. 1975;42:193-226 PMID: 236638
  6. Effect of denaturation on the susceptibility of proteins to enzymic phosphorylation.
    J Biol Chem. 1975 Aug 25;250(16):6355-61 PMID: 169238
  7. Studies on the inhibition of hepatic lipogenesis by N-6,O-2'-dibutyryl adenosine 3',5'-monophosphate.
    Arch Biochem Biophys. 1975 Jul;169(1):168-80 PMID: 169739
  8. Functional consequences of modifying highly reactive arginyl residues of fructose 1,6-bisphosphatase. Loss of monovalent cation activation.
    Biochemistry. 1975 Aug 26;14(17):3916-21 PMID: 169892
  9. Hepatic metabolism of genetically diabetic (db/db) mice. I. Carbohydrate metabolism.
    Am J Physiol. 1975 Dec;229(6):1702-12 PMID: 174448
  10. Exploitable molecular mechanisms in hibernation--I. Liver diphosphofructose phosphatase of the rat and hamster: a comparison.
    Comp Biochem Physiol B. 1976;55(2):283-91 PMID: 9263
  11. The purification of properties of rat liver fructose 1,6-bisphosphatase.
    Arch Biochem Biophys. 1976 Nov;177(1):253-64 PMID: 11750
  12. Purification and characterization of the catalytic subunit of adenosine 3':5'-cyclic monophosphate-dependent protein kinase from bovine liver.
    Biochem J. 1976 Nov;159(2):409-22 PMID: 187175
  13. Hormonal control of [14C]glucose synthesis from [U-14C]dihydroxyacetone and glycerol in isolated rat hepatocytes.
    J Biol Chem. 1976 Dec 25;251(24):7841-52 PMID: 187597
  14. Effects of hormones and of ethanol on the fructose 6-P-fructose 1,6-P2 futile cycle during gluconeogenesis in the liver.
    Arch Biochem Biophys. 1976 Dec;177(2):337-45 PMID: 1015825
  15. Improved resolution of myofibrillar proteins with sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
    Biochim Biophys Acta. 1977 Jan 25;490(1):27-34 PMID: 836873
  16. In vivo and in vitro phosphorylation of rat liver fructose-1,6-bisphosphatase.
    Proc Natl Acad Sci U S A. 1977 Oct;74(10):4615-9 PMID: 200922
  17. Effect of glucagon administration on mice liver fructose-1, 6-bisphosphatase.
    Biochem Biophys Res Commun. 1978 Oct 30;84(4):950-6 PMID: 31877
  18. Hormonal control of hepatic gluconeogenesis.
    Vitam Horm. 1978;36:383-460 PMID: 217173
  19. The synthesis and degradation of rat liver and kidney fructose bisphosphatase in vivo.
    Arch Biochem Biophys. 1979 May;194(2):620-31 PMID: 220919
  20. Chemical and regulatory properties of phosphorylase kinase and cyclic AMP-dependent protein kinase.
    Adv Enzymol Relat Areas Mol Biol. 1979;50:41-115 PMID: 227235
  21. Amino acid sequence at the phosphorylated site of rat liver fructose-1,6-diphosphatase and phosphorylation of a corresponding synthetic peptide.
    Biochem Biophys Res Commun. 1979 Oct 12;90(3):1064-72 PMID: 228666
  22. Regulatory functions of protein multisite phosphorylation.
    Mol Cell Endocrinol. 1979 Dec;16(3):157-79 PMID: 230103
  23. Purification and properties of liver fructose 1,6-bisphosphatase from C57BL/KsJ normal and diabetic mice.
    J Biol Chem. 1980 Mar 25;255(6):2481-6 PMID: 6244280
  24. Influence of glucagon on the metabolism of xylitol and dihydroxyacetone in the isolated perfused rat liver.
    J Biol Chem. 1973 May 25;248(10):3601-7 PMID: 4349871
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.1.91
Published
1981-01-00
Pages
91-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC318996
Subset
IM
Grants
NIADDK NIH HHS · AM 21167 · United States
NHLBI NIH HHS · HL 23306 · United States
NCRR NIH HHS · RR 05366 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.1.91 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2