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PMID: 6264147 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of unintegrated forms of Kirsten murine sarcoma viral DNA and restriction endonuclease cleavage map of linear DNA.

Journal of virology ·Vol. 38 ·No. 2 ·1981-05-00 ·Pages 797-803

Tsuchida N, Kominami R, Hatanaka M, Uesugi S

Abstract

We detected unintegrated linear 7.0-kilobase pair DNA and covalently closed circular DNA species in NIH3T3 cells recently infected with Kirsten murine sarcoma virus. Using the linear DNA, we constructed a restriction endonuclease cleavage map and compared it with the map of Harvey murine sarcoma virus. The restriction endonuclease maps of two segments, one 1.2 kilobase pairs (SmaI site) to 3.7 kilobase pairs (HindIII site) from the right end (corresponding to the viral 3' side) and the other 0.5 kilobase pair (SmaI and KpnI sites) to 0.9 kilobase pair (KpnI site) from the left end, were identical in the two virus types.

MeSH Terms
Animals Base Sequence Cell Line DNA Restriction Enzymes DNA, Circular DNA, Viral Deoxyribonuclease HindIII Deoxyribonucleases, Type II Site-Specific Genes, Viral Kirsten murine sarcoma virus/genetics Mice Recombination, Genetic Sarcoma Viruses, Murine/genetics
Chemicals
DNA, Circular DNA, Viral DNA Restriction Enzymes Deoxyribonuclease HindIII CCCGGG-specific type II deoxyribonucleases Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tsuchida N
Kominami R
Hatanaka M
Uesugi S
References (35)
35 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.38.2.797-803.1981
Published
1981-05-00
Pages
797-803
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC171212
Subset
IM
Grants
NCI NIH HHS · CA-21124 · United States
NCI NIH HHS · CA-22701 · United States
External Links
PubMed source DOI: 10.1128/JVI.38.2.797-803.1981 PMC Full Text View journal details
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