Home LiteratureArticle Details
PMID: 6264142 Published · ppublish English Comparative Study Journal Article

Analysis of JC virus DNA purified directly from human progressive multifocal leukoencephalopathy brains.

Journal of virology ·Vol. 38 ·No. 2 ·1981-05-00 ·Pages 761-9

Rentier-Delrue F, Lubiniecki A, Howley PM

Abstract

Human polyomavirus JC DNA was purified directly from the diseased brain tissue of two patients with progressive multifocal leukoencephalopathy (PML) by a method employing differential salt precipitation (B. Hirt, J. Mol. Biol. 26:365-369, 1967). Each of the viral genomes (JC-NIH-1 and JC-NIH-2) was molecularly cloned intact in Escherichia coli, using pBR322, at their unique EcoRI (0.00 map unit) and BamHI (0.51 map unit) sites. The JC-NIH-1 genome was approximately 50 base pairs larger and the JC-NIH-2 genome was approximately 50 base pairs smaller than the prototype human polyomavirus JC (Mad-1) DNA. Analysis of the restriction endonuclease cleavage fragments of these two DNAs and the human polyomavirus JC (Mad-1) DNA revealed only slight differences which mapped in a region of the genome extending from 0.67 to 0.74 map unit. From previous homology studies, this region of variance corresponds to the noncoding region to the late side of the origin of DNA replication.

MeSH Terms
Brain/microbiology Cloning, Molecular DNA Restriction Enzymes DNA, Viral/analysis,isolation & purification Genes, Viral Humans Leukoencephalopathy, Progressive Multifocal/microbiology Male Middle Aged Polyomavirus/genetics
Chemicals
DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rentier-Delrue F
Lubiniecki A
Howley P M
References (37)
37 references, click to expand
  1. Selective extraction of polyoma DNA from infected mouse cell cultures.
    J Mol Biol. 1967 Jun 14;26(2):365-9 PMID: 4291934
  2. A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.
    Proc Natl Acad Sci U S A. 1967 May;57(5):1514-21 PMID: 5231757
  3. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.
    J Bacteriol. 1972 May;110(2):667-76 PMID: 4336693
  4. Prevalence of antibodies in human sera against JC virus, an isolate from a case of progressive multifocal leukoencephalopathy.
    J Infect Dis. 1973 Apr;127(4):467-70 PMID: 4571704
  5. Comparison of JC and BK human papovaviruses with simian virus 40: restriction endonuclease digestion and gel electrophoresis of resultant fragments.
    J Virol. 1974 Mar;13(3):614-22 PMID: 4362864
  6. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  7. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  8. Integrated simian virus 40 sequences in transformed cell DNA: analysis using restriction endonucleases.
    Proc Natl Acad Sci U S A. 1976 Apr;73(4):1102-6 PMID: 177975
  9. Polynucleotide sequences common to the genomes of simian virus 40 and the human papovaviruses JC and BK.
    Virology. 1976 Aug;73(1):303-7 PMID: 183358
  10. New human papovaviruses.
    Prog Med Virol. 1976;22:1-35 PMID: 62371
  11. Common structural antigen of papovaviruses of the simian virus 40-polyoma subgroup.
    J Virol. 1977 Jan;21(1):179-86 PMID: 64625
  12. JC virus, a human polyomavirus associated with progressive multifocal leukoencephalopathy: additional biological characteristics and antigenic relationships.
    Infect Immun. 1977 Feb;15(2):656-62 PMID: 191404
  13. Mapping of the HhaI and HinfI cleavage sites on simian virus 40 DNA.
    J Mol Biol. 1977 Feb 25;110(2):297-317 PMID: 191618
  14. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  15. Polyoma virus in urine during pregnancy.
    Lancet. 1977 Oct 1;2(8040):709-10 PMID: 71513
  16. Electron microscope study of the base sequence homology between simian virus 40 and human papovavirus BK.
    J Virol. 1978 Jan;25(1):193-201 PMID: 202735
  17. Nucleotide sequence of the DNA replication origin for human papovavirus BKV: sequence and structural homology with SV40.
    Cell. 1978 Feb;13(2):345-58 PMID: 203403
  18. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  19. The genome of simian virus 40.
    Science. 1978 May 5;200(4341):494-502 PMID: 205947
  20. pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long.
    Nucleic Acids Res. 1978 Aug;5(8):2721-8 PMID: 358147
  21. Detection of polyoma virus DNA in PML-brain tissue by (in situ) hybridization.
    J Gen Virol. 1979 Jan;42(1):49-57 PMID: 215712
  22. Molecular cloning of polyoma virus DNA in Escherichia coli: plasmid vector system.
    Science. 1979 Mar 2;203(4383):883-7 PMID: 217087
  23. Preparative and analytical purification of DNA from agarose.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):615-9 PMID: 284385
  24. A rapid method for detecting and mapping homology between heterologous DNAs. Evaluation of polyomavirus genomes.
    J Biol Chem. 1979 Jun 10;254(11):4876-83 PMID: 220264
  25. Restriction endonuclease cleavage map of the DNA of JC virus.
    J Virol. 1979 Mar;29(3):846-55 PMID: 221677
  26. Mapping and ordering of fragments of BK virus DNA produced by restriction endonucleases.
    J Virol. 1979 Mar;29(3):915-25 PMID: 221680
  27. The colinear alignment of the genomes of papovaviruses JC, BK, and SV40.
    Virology. 1979 Jul 30;96(2):576-87 PMID: 223318
  28. JC human papovavirus replication in human amnion cells.
    J Virol. 1979 Apr;30(1):384-9 PMID: 480459
  29. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
  30. Infectivity of the DNA from four isolates of JC virus.
    J Virol. 1979 Nov;32(2):476-82 PMID: 228071
  31. BK virus DNA: complete nucleotide sequence of a human tumor virus.
    Science. 1979 Oct 26;206(4417):456-62 PMID: 228391
  32. Human polyomavirus infections with JC virus and BK virus in renal transplant patients.
    Ann Intern Med. 1980 Mar;92(3):373-8 PMID: 6243896
  33. Rapid detection and identification of JC virus and BK virus in human urine by using immunofluorescence microscopy.
    J Clin Microbiol. 1980 Feb;11(2):178-83 PMID: 6244330
  34. Growth of JC virus in adult human brain cell cultures.
    Arch Virol. 1980;65(2):141-8 PMID: 7425855
  35. Characterization of JC papovavirus adapted to growth in human embryonic kidney cells.
    J Virol. 1980 Aug;35(2):498-504 PMID: 6255188
  36. Cloned human polyomavirus JC DNA can transform human amnion cells.
    J Virol. 1980 Dec;36(3):878-82 PMID: 6257931
  37. Cultivation of papova-like virus from human brain with progressive multifocal leucoencephalopathy.
    Lancet. 1971 Jun 19;1(7712):1257-60 PMID: 4104715
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.38.2.761-769.1981
Published
1981-05-00
Pages
761-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC171206
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.38.2.761-769.1981 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2