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PMID: 202735 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Electron microscope study of the base sequence homology between simian virus 40 and human papovavirus BK.

Journal of virology ·Vol. 25 ·No. 1 ·1978-01-00 ·Pages 193-201

Newell N, Lai CJ, Khoury G, Kelly TJ

Abstract

The base sequence homology between the genomes of simian virus 40 (SV40) and human papovavirus BK (BKV) was studied by the heteroduplex method of Ferguson and Davis (J. Mol. Biol. 94:135-149, 1975). When mounted for microscopy in 30% formamide (Tm-35 degrees C), BKV/SV40 heteroduplexes were an average of 92% double-stranded and contained only two small nonhomologous regions that mapped near the junctions between the early and late regions of the SV40 Genome. At higher formamide concentrations, the fraction of duplex DNA in the BKV/SV40 heteroduplexes decreased, indicating significant base mismatching in the homologous regions. The strongest regions of homology were located in the late region.

MeSH Terms
BK Virus/analysis Base Sequence DNA, Viral/analysis Microscopy, Electron Nucleic Acid Conformation Polyomavirus/analysis Simian virus 40/analysis Temperature
Chemicals
DNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Newell N
Lai C J
Khoury G
Kelly T J
References (38)
38 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.25.1.193-201.1978
Published
1978-01-00
Pages
193-201
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353916
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.25.1.193-201.1978 PMC Full Text View journal details
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