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PMID: 6264127 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Organization of viral genome in a T antigen-negative hamster tumor induced by human papovavirus BK.

Journal of virology ·Vol. 38 ·No. 2 ·1981-05-00 ·Pages 556-63

Yogo Y, Furuno A, Nozawa A, Uchida S

Abstract

We analyzed by blot hybridization the state and structure of the viral DNA in an exceptional BK virus-induced hamster tumor (choroid plexus papilloma Vn-324) that contains about one copy of the BK virus genome per cell, but no intranuclear T antigen as assayed by indirect immunofluorescence. The BK viral DNA was found to be integrated into cellular DNA at a site in the middle of the early region of the viral genome (between 0.32 and 0.41 map units). The structure of the inserted viral DNA shows that it cannot encode a full-size large T antigen, but may encode small T antigen and an N-terminal portion of large T antigen.

MeSH Terms
Animals Antigens, Neoplasm/analysis Antigens, Viral/analysis Antigens, Viral, Tumor BK Virus/genetics Base Sequence Cerebral Ventricle Neoplasms/microbiology Choroid Plexus Cricetinae DNA, Viral/genetics Genes, Viral Nucleic Acid Hybridization Polyomavirus/genetics Recombination, Genetic Tumor Virus Infections/microbiology
Chemicals
Antigens, Neoplasm Antigens, Viral Antigens, Viral, Tumor DNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yogo Y
Furuno A
Nozawa A
Uchida S
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26 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.38.2.556-563.1981
Published
1981-05-00
Pages
556-63
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC171186
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.38.2.556-563.1981 PMC Full Text View journal details
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