Home LiteratureArticle Details
PMID: 6246489 Published · ppublish English Journal Article

Polyoma large tumor antigen is not required for tumorigenesis mediated by viral DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 3 ·1980-03-00 ·Pages 1336-40

Moore JL, Chowdhury K, Martin MA, Israel MA

Abstract

The arrangement of viral DNA sequences in a hamster cell line derived from a tumor induced by a recombinant plasmid DNA preparation containing the entire polyoma virus genome was examined. In the recombinant plasmid employed, viral DNA sequences specifying the large species of polyoma tumor antigen but not the small and middle tumor antigens were interrupted by the insertion of plasmid DNA at the EcoRI restriction endonuclease site. Blot-hybridization analyses of tumor cell DNA indicated that the "joints" linking viral and plasmid DNAs in the original recombinant plasmid used in animal inoculation had been preserved. Integration into the hamster cell genome had apparently occurred within plasmid DNA sequences. These results indicate that polyoma large tumor antigen is not required for tumorigenesis mediated by viral DNA.

MeSH Terms
Animals Antigens, Neoplasm/genetics Cell Line Cell Transformation, Neoplastic Cell Transformation, Viral Cricetinae DNA, Recombinant DNA, Viral/genetics Genes, Viral Molecular Weight Plasmids Polyomavirus/genetics,immunology Viral Proteins/genetics
Chemicals
Antigens, Neoplasm DNA, Recombinant DNA, Viral Viral Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Moore J L
Chowdhury K
Martin M A
Israel M A
References (30)
30 references, click to expand
  1. Complementation and transformation by temperature-sensitive mutants of polyoma virus.
    Virology. 1969 May;38(1):120-5 PMID: 4306521
  2. Temperature-sensitive mutants of polyoma virus.
    Virology. 1969 May;38(1):126-33 PMID: 4306522
  3. Host range mutants of polyoma virus.
    Proc Natl Acad Sci U S A. 1970 Sep;67(1):394-9 PMID: 4318787
  4. Nucleotide sequence of the rightward operator of phage lambda.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8 PMID: 1055375
  5. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  6. Integrated simian virus 40 sequences in transformed cell DNA: analysis using restriction endonucleases.
    Proc Natl Acad Sci U S A. 1976 Apr;73(4):1102-6 PMID: 177975
  7. Construction of the genetic map of the polyoma genome.
    J Virol. 1976 Jun;18(3):824-32 PMID: 178924
  8. The arrangement of simian virus 40 sequences in the DNA of transformed cells.
    Cell. 1976 Oct;9(2):269-87 PMID: 184963
  9. Localization of gene functions in polyoma virus DNA.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):4169-73 PMID: 186787
  10. Characterization of polyoma virus T antigen.
    Proc Natl Acad Sci U S A. 1977 Mar;74(3):1259-63 PMID: 191834
  11. Complementation between temperature-sensitive (ts) and host range nontransforming (hr-t) mutants of polyoma virus.
    Virology. 1977 Apr;77(2):589-97 PMID: 193254
  12. Hr-t and ts-a: two early gene functions of polyoma virus.
    Virology. 1977 Apr;77(2):610-24 PMID: 193256
  13. A physical map of the DNA regions flanking the rabbit beta-globin gene.
    Cell. 1977 Oct;12(2):429-39 PMID: 912752
  14. Temperature-sensitive growth regulation in one type of transformed rat cells induced by the tsa mutant of polyoma virus.
    J Virol. 1977 Dec;24(3):721-8 PMID: 201774
  15. Tumor antigen(s) in cell productively infected by wild-type polyoma virus and mutant NG-18.
    Proc Natl Acad Sci U S A. 1978 Jan;75(1):79-83 PMID: 203944
  16. Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9.
    Gene. 1977;2(2):75-93 PMID: 344136
  17. Characterization of T antigens in polyoma-infected and transformed cells.
    Cell. 1978 Sep;15(1):65-77 PMID: 212199
  18. Conditions leading to the establishment of the N (a gene dependent) and A (a gene independent) transformed states after polyoma virus infection of rat fibroblasts.
    J Virol. 1978 Nov;28(2):421-6 PMID: 214570
  19. Evaluation of normal and neoplastic human tissue for BK virus.
    Virology. 1978 Oct 15;90(2):187-96 PMID: 214941
  20. Three species of polyoma virus tumor antigens share common peptides probably near the amino termini of the proteins.
    Cell. 1978 Dec;15(4):1427-37 PMID: 215327
  21. Molecular cloning of polyoma virus DNA in Escherichia coli: plasmid vector system.
    Science. 1979 Mar 2;203(4383):883-7 PMID: 217087
  22. Multiple forms of polyoma virus tumor antigens from infected and transformed cells.
    J Virol. 1979 Mar;29(3):881-7 PMID: 221679
  23. Biological activity of polyoma viral DNA in mice and hamsters.
    J Virol. 1979 Mar;29(3):990-6 PMID: 221686
  24. Sequence from early region of polyoma virus DNA containing viral replication origin and encoding small, middle and (part of) large T antigens.
    Cell. 1979 Jun;17(2):357-70 PMID: 222480
  25. Molecular cloning of polyoma virus DNA in Escherichia coli: oncogenicity testing in hamsters.
    Science. 1979 Sep 14;205(4411):1140-2 PMID: 224458
  26. The nucleotide sequence and genome organization of the polyoma early region: extensive nucleotide and amino acid homology with SV40.
    Cell. 1979 Jul;17(3):715-24 PMID: 225042
  27. Polyoma virus-specific 55K protein isolated from plasma membrane of productively infected cells is virus-coded and important for cell transformation.
    Virology. 1979 Oct 15;98(1):261-6 PMID: 225866
  28. Interrupting the early region of polyoma virus DNA enhances tumorigenicity.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3713-6 PMID: 226975
  29. New classes of viable deletion mutants in the early region of polyoma virus.
    J Virol. 1979 Sep;31(3):645-56 PMID: 229246
  30. CELL-TRANSFORMING ABILITY OF A TEMPERATURE-SENSITIVE MUTANT OF POLYOMA VIRUS.
    Proc Natl Acad Sci U S A. 1965 Mar;53:486-91 PMID: 14338226
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.3.1336
Published
1980-03-00
Pages
1336-40
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC348489
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.3.1336 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2