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PMID: 6263791 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Primary culture of capillary endothelium from rat brain.

In vitro ·Vol. 17 ·No. 4 ·1981-04-00 ·Pages 353-62

Bowman PD, Betz AL, Ar D, Wolinsky JS, Penney JB, Shivers RR, Goldstein GW

Abstract

To provide an in vitro system for studies of brain capillary function we developed a method for culture of brain capillary endothelial cells. Capillaries were isolated from rat brain and enzymatically treated to remove the basement membrane and contaminating pericytes. Subsequent Percoll gradient centrifugation resulted in a homogeneous population of capillary endothelial cells that attached to a collagen substrate and incorporated [3H]thymidine. Evidence for the endothelial nature of these cells was provided by the presence of Factor VIII antigen and angiotensin converting enzyme activity and by the failure of platelets to adhere to the cell surface. In addition, the cells were joined together by tight junctions. Thus, primary cultures of these cells retained both endothelial and blood-brain barrier features.

MeSH Terms
Animals Antigens/analysis Blood-Brain Barrier Brain/blood supply Capillaries Cell Division Cell Separation Cells, Cultured Endothelium/cytology Factor VIII/analysis,immunology Intercellular Junctions/ultrastructure Organoids/ultrastructure Peptidyl-Dipeptidase A/metabolism Pinocytosis Rats von Willebrand Factor
Chemicals
Antigens von Willebrand Factor Factor VIII Peptidyl-Dipeptidase A
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Bowman P D
Betz A L
Ar D
Wolinsky J S
Penney J B
Shivers R R
Goldstein G W
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Article Info
Journal
In vitro
Abbr.
In Vitro
ISSN
0073-5655
DOI
10.1007/BF02618147
Published
1981-04-00
Pages
353-62
Language
English
Region
United States
NLM ID
0063733
Subset
IM
Grants
NIEHS NIH HHS · ES-02380 · United States
NHLBI NIH HHS · HL-25492 · United States
NINDS NIH HHS · NS-00443 · United States
External Links
PubMed source DOI: 10.1007/BF02618147
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