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PMID: 620753 Published · ppublish English Journal Article

Evidence for the presence of viable endothelial cells in cultures derived from dissociated rat brain.

Experientia ·Vol. 34 ·No. 1 ·1978-01-15 ·Pages 95-7

Panula P, Joó F, Rechardt L

Abstract

The morphology and histochemistry of dissociated newborn rat brain was studied in tissue culture. Direct microscopy of developing cells, electron microscopy and the alkaline phosphatase activity were used to identify the capillary endothelial cells.

MeSH Terms
Alkaline Phosphatase/analysis Animals Animals, Newborn Brain/cytology Brain Chemistry Culture Techniques Endothelium/ultrastructure Histocytochemistry Rats
Chemicals
Alkaline Phosphatase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Panula P
Joó F
Rechardt L
References (6)
6 references, click to expand
  1. Observations on closed tissue cultures of sympathetic ganglia of chick embryos in media buffered with N-tris-(hydroxymethyl) methyl-glycine or N-2-hydroxy-ethylpiperazine-N-2-ethanesulfonic acid.
    Acta Physiol Scand. 1974 Jan;90(1):267-77 PMID: 4360702
  2. The localization of monoaminergic blood-brain barrier mechanisms.
    Pharmacol Rev. 1966 Mar;18(1):369-85 PMID: 5904153
  3. Histochemical studies on the phosphatase of the nervous system.
    J Comp Neurol. 1950 Oct;93(2):201-17 PMID: 14784516
  4. A procedure for the isolation of capillaries from rat brain.
    Cytobios. 1973 Sep-Oct;8(29):41-8 PMID: 4774116
  5. Interactions of neurons and astrocytes during growth and development of human fetal brain in vitro.
    Exp Mol Pathol. 1976 Feb;24(1):110-25 PMID: 767131
  6. Neurons and glia in neural cultures.
    Exp Neurol. 1975 Sep;48(3 pt. 2):93-195 PMID: 171169
Article Info
Journal
Experientia
Abbr.
Experientia
ISSN
0014-4754
Published
1978-01-15
Pages
95-7
Language
English
Region
Switzerland
NLM ID
0376547
Subset
IM
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