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PMID: 6262736 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Comparison of the nucleoside sequence of trpA and sequences immediately beyond the trp operon of Klebsiella aerogenes. Salmonella typhimurium and Escherichia coli.

Nucleic acids research ·Vol. 9 ·No. 7 ·1981-04-10 ·Pages 1743-55

Nichols BP, Blumenberg M, Yanofsky C

Abstract

The nucleotide sequence of trpA of Klebsiella aerogenes is presented and compared with the trpA sequences of Salmonella typhimurium and Escherichia coli. The majority of the approximately 200 differences between each pair of trpA's are single nucleotide pair changes that do not alter the amino acid sequence. Codon usage conforms to the general patterns revealed by examination of other prokaryotic gene sequences. However, codon usage in K. aerogenes trpA reflects the high G+C content of the genome of this organism. The DNA sequences just beyond trpA, the presumed transcription termination region, are also compared for the three species. Perusal of these sequences indicates that the secondary structure of the transcript segment just beyond trpA has been preserved, while the primary sequence has diverged appreciably.

MeSH Terms
Amino Acid Sequence Base Sequence Codon DNA Restriction Enzymes DNA, Bacterial Escherichia coli/genetics Genes Klebsiella pneumoniae/genetics Nucleic Acid Conformation Operon Salmonella typhimurium/genetics Species Specificity Transcription, Genetic
Chemicals
Codon DNA, Bacterial DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nichols B P
Blumenberg M
Yanofsky C
References (18)
18 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
Published
1981-04-10
Pages
1743-55
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326794
Subset
IM
Grants
NIGMS NIH HHS · GM09738 · United States
Databases
GENBANK
J01738
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