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PMID: 4113124 Published · ppublish English Journal Article

Enzyme evolution in the Enterobacteriaceae.

Journal of bacteriology ·Vol. 110 ·No. 3 ·1972-06-00 ·Pages 793-802

Cocks GT, Wilson AC

Abstract

An immunological approach has been used for the study of alkaline phosphatase evolution in bacteria of the family Enterobacteriaceae. Antisera were prepared against alkaline phosphatase from Escherichia coli and Klebsiella aerogenes and tested against the unpurified alkaline phosphatases of 32 strains of enterobacteria by double diffusion and quantitative micro-complement fixation. The immunological relationships detected among the alkaline phosphatases of enterobacteria agree approximately with those reported for five other enzymes, as well as with the tryptic peptide pattern similarities found for two other enzymes, and with the relationships detected by interspecific deoxyribonucleic acid hybridization tests.

MeSH Terms
Alkaline Phosphatase/analysis Amino Acid Sequence Animals Biological Evolution Chromatography, DEAE-Cellulose Chromatography, Gel Complement Fixation Tests Cross Reactions DNA, Bacterial/analysis Electrophoresis, Disc Enterobacteriaceae/classification,enzymology,immunology Epitopes Erwinia/enzymology Escherichia coli/enzymology Immune Sera Immunodiffusion Klebsiella/enzymology Male Proteus/enzymology Rabbits/immunology Salmonella/enzymology Serratia/enzymology Shigella/enzymology
Chemicals
DNA, Bacterial Epitopes Immune Sera Alkaline Phosphatase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cocks G T
Wilson A C
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41 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
Published
1972-06-00
Pages
793-802
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC247495
Subset
IM
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