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PMID: 6262724 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterisation of the genes for ribosomal RNA in flax.

Nucleic acids research ·Vol. 9 ·No. 6 ·1981-03-25 ·Pages 1301-9

Goldsbrough PB, Cullis CA

Abstract

DNA coding for the 18S and 25S rRNAs in flax (Linum usitatissimum) has been purified and is arranged in tandem arrays with a repeat length of 8.6 kb. There is no detectable variation in the size of this repeat unit. Single repeat units have been cloned in the plasmid pAT 153. The coding sequences for the 25S, 18s and 5.85 rRNAs have been localised by hybridisation. The cloned rDNA has been used to compare two genotrophs, L1 ad S1, where the number of rRNA cistrons has been altered by growth under different environmental conditions. In terms of the size of the repeat unit and the position of a number of restriction enzyme sites the rDNAs from L1 and S1 were identical.

MeSH Terms
Cloning, Molecular DNA/genetics DNA Restriction Enzymes Genes Molecular Weight Nucleic Acid Hybridization Plants/genetics RNA, Ribosomal/genetics Repetitive Sequences, Nucleic Acid
Chemicals
RNA, Ribosomal DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Goldsbrough P B
Cullis C A
References (11)
11 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.6.1301
Published
1981-03-25
Pages
1301-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326758
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/9.6.1301 PMC Full Text View journal details
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