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PMID: 6262520 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Two major DNA variants present in serially propagated stocks of the WR strain of vaccinia virus.

Journal of virology ·Vol. 37 ·No. 3 ·1981-03-00 ·Pages 1000-10

Panicali D, Davis SW, Mercer SR, Paoletti E

Abstract

Two major DNA variants have been isolated from serially propagated stocks of the WR strain of vaccinia virus. Restriction enzyme mapping of the two variants with HindIII, AvaI, XhoI, SstII, and SmaI revealed a 6.3-megadalton deletion in the smaller DNA variant. The deletion was mapped at ca. 6.8 megadaltons in from the left terminus, just beyond the inverted terminal repeat. The additional DNA present in the larger variant was found to represent unique viral sequences that were transcribed both in vitro and in vivo. One-step growth curves in HeLa cells revealed no difference in rat of replication or burst size when progeny was scored on CV-1 monolayers.

MeSH Terms
Base Sequence DNA Restriction Enzymes DNA, Viral Deoxyribonuclease HindIII Genes, Viral HeLa Cells Humans Nucleic Acid Hybridization Vaccinia virus/genetics,growth & development
Chemicals
DNA, Viral DNA Restriction Enzymes Deoxyribonuclease HindIII
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Panicali D
Davis S W
Mercer S R
Paoletti E
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23 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.37.3.1000-1010.1981
Published
1981-03-00
Pages
1000-10
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC171098
Subset
IM
Grants
NIGMS NIH HHS · GM-23853 · United States
External Links
PubMed source DOI: 10.1128/JVI.37.3.1000-1010.1981 PMC Full Text View journal details
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