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PMID: 7365867 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Capped and polyadenylated low-molecular-weight RNA synthesized by vaccinia virus in vitro.

Journal of virology ·Vol. 33 ·No. 1 ·1980-01-00 ·Pages 208-19

Paoletti E, Lipinskas BR, Panicali D

Abstract

In the presence of ATP plus two other ribonucleoside triphosphates or in reactions containing all four ribonucleoside triphosphates and actinomycin D, vaccinia virus synthesizes in vitro discrete low-molecular-weight RNA molecules ranging in size from about 20 to several hundred bases. A novel feature of these small RNA molecules is that they are capped and methylated at the 5' terminus, containing both mGpppGm and mGpppAm type cap structures, and in addition these molecules are polyadenylated at the 3' terminus. Hybridization of these RNAs to restriction fragments derived from vaccinia virus DNA indicates a considerable degree of complexity, suggesting the presence of a large number of promoters throughout the genome. However, measurable sensitivity to pancreatic RNase of the 5' capped end of these RNAs while in hybrid form to the DNA suggests other possible roles for these small RNAs in vaccinia virus mRNA biogenesis.

MeSH Terms
Adenosine Triphosphate/metabolism Base Composition Cell-Free System DNA-Directed RNA Polymerases/metabolism Molecular Weight Poly A/analysis RNA Caps/biosynthesis RNA, Viral/analysis,biosynthesis Ribonucleotides/metabolism S-Adenosylmethionine/metabolism Vaccinia virus/analysis,metabolism
Chemicals
RNA Caps RNA, Viral Ribonucleotides Poly A S-Adenosylmethionine Adenosine Triphosphate DNA-Directed RNA Polymerases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Paoletti E
Lipinskas B R
Panicali D
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35 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.33.1.208-219.1980
Published
1980-01-00
Pages
208-19
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC288537
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.33.1.208-219.1980 PMC Full Text View journal details
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