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PMID: 6262331 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Coordinate, equimolar secretion of smaller peptide products derived from pro-ACTH/endorphin by mouse pituitary tumor cells.

The Journal of cell biology ·Vol. 89 ·No. 1 ·1981-04-00 ·Pages 21-8

Mains RE, Eipper BA

Abstract

The secretion of peptide products derived from pro-ACTH/endorphin was examined with several radioimmunoassays and with polyacrylamide gel analyses of immunoprecipitates of radioactively labeled peptides. In studies using a mouse pituitary tumor cell line the accumulation of each of the four molecular forms of adrenocorticotropic hormone (ACTH) in tissue culture medium was shown to be a linear function of time. No evidence for self inhibition of secretion by accumulated, secreted peptides (i.e., ultra-short feedback) was found. Furthermore, synthetic human ACTH and synthetic camel beta-endorphin did not alter secretion of peptides when added to the culture medium at levels up to 10,000 times physiological. Stimulation of the release of ACTH-, endorphin-, lipotropin-, and 16k fragment immunoreactive material by norepinephrine was fully blocked by cobalt; by this criterion, stimulated release was calcium dependent. All the smaller molecules derived from the pro-ACTH/endorphin common precursor were secreted in equimolar amounts under all circumstances tested, within the precision of these studies (+/- 11%). Norepinephrine and cobalt did not significantly alter the secretion of pro-ACTH/endorphin and ACTH biosynthetic intermediate. The stimulation of secretion by norepinephrine and inhibition of secretion by cobalt was restricted to the lower molecular weight products derived from pro-ACTH/endorphin: glycosylated and nonglycosylated ACTH(1-39); beta-lipotropin, beta-endorphin, and gamma-lipotropin; and 16k fragment.

MeSH Terms
Adrenocorticotropic Hormone/biosynthesis,metabolism Animals Cell Line Endorphins/metabolism Kinetics Mice Neoplasms, Experimental/metabolism Peptides/metabolism Pituitary Hormones, Anterior/metabolism Pituitary Neoplasms/metabolism Pro-Opiomelanocortin Protein Precursors/metabolism beta-Lipotropin/metabolism
Chemicals
Endorphins Peptides Pituitary Hormones, Anterior Protein Precursors Pro-Opiomelanocortin Adrenocorticotropic Hormone beta-Lipotropin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mains R E
Eipper B A
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57 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.89.1.21
Published
1981-04-00
Pages
21-8
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2111776
Subset
IM
Grants
NIADDK NIH HHS · AM-18929 · United States
NIADDK NIH HHS · AM-19859 · United States
External Links
PubMed source DOI: 10.1083/jcb.89.1.21 PMC Full Text View journal details
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