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PMID: 6258163 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Breakage of single-stranded DNA by rat liver nicking-closing enzyme with the formation of a DNA-enzyme complex.

Nucleic acids research ·Vol. 8 ·No. 24 ·1980-12-20 ·Pages 6129-42

Been MD, Champoux JJ

Abstract

The DNA nicking-closing enzyme (type I topoisomerase) from rat liver nuclei breaks single-stranded DNA. The broken strand contains a 5'-hydroxyl and tightly bound protein. The stability of this protein-DNA complex to high salt, alkali and detergent suggests a covalent linkage between the DNA and the enzyme. The observed breakage of single-stranded DNA occurs at neutral pH prior to treatment with alkali or detergent, indicating that the breakage may be the result of an interrupted nicking and closing cycle. The resulting covalent complex could represent a reaction intermediate in the overall nicking-closing reaction.

MeSH Terms
Animals Bacteriophage phi X 174 DNA Topoisomerases, Type I/metabolism DNA, Single-Stranded/metabolism DNA, Viral/metabolism Kinetics Liver/enzymology Protein Binding Rats Ultracentrifugation
Chemicals
DNA, Single-Stranded DNA, Viral DNA Topoisomerases, Type I
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Been M D
Champoux J J
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20 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.24.6129
Published
1980-12-20
Pages
6129-42
Language
English
Region
England
NLM ID
0411011
PMCID
PMC328077
Subset
IM
Grants
NIGMS NIH HHS · 1-T32-GM-07270 · United States
NIGMS NIH HHS · GM23224 · United States
External Links
PubMed source DOI: 10.1093/nar/8.24.6129 PMC Full Text View journal details
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