Physical mapping of the BglI, BglII, PstI and EcoRI restriction fragments of staphylococcal phage phi 11 DNA.

Molecular & general genetics : MGG ·Vol. 180 ·No. 2 ·1980-00-00 ·Pages 391-8

Bächi B

Abstract

A cleavage map of the generalized transducing staphylococcal phage phi 11 DNA has been constructed by reciprocal double digestion. All three BglI, the six BglII, the three PstI, and 11 out of 15 EcoRI sites have been mapped. The map is circular, with a total length of 42 kb, and has been divided into 100 map units. The phage DNA is cyclically permuted and has a terminal redundancy of about 11 kb. The preferential starting point and direction for packaging DNA lies at map unit 79 and proceeds towards higher map units.

MeSH Terms

Bacterial Proteins Base Sequence Chromosome Mapping DNA Restriction Enzymes DNA, Viral Deoxyribonucleases, Type II Site-Specific Staphylococcus Phages/genetics

Chemicals

Bacterial Proteins DNA, Viral DNA Restriction Enzymes BglII endonuclease CTGCAG-specific type II deoxyribonucleases Deoxyribonucleases, Type II Site-Specific GCCNNNNNGGC-specific type II deoxyribonucleases

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Bächi B

References (15)

15 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00425853

Published

1980-00-00

Pages

391-8

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00425853

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Physical mapping of the BglI, BglII, PstI and EcoRI restriction fragments of staphylococcal phage phi 11 DNA.

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